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Food Science and Human Wellness 2024, 13(2): 909-919 https://doi.org/10.26599/FSHW.2022.9250078
Open Access | Issue | Published: 25 September 2023

Biochemistry and transcriptome analysis reveal condensed tannins alleviate liver injury induced by regulating cholesterol metabolism pathway

Show Author's Information Xiangxin Lia,1,  Yijing Pua,b,1,  Bangdi Liuc,  Xiaoming Fanga,  Wenjun Penga,  Weibo Jiangb( ),  Wenli Tiana,( )
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
Academy of Agricultural Planning and Engineering, Ministry of Agriculture and Rural Affairs, Beijing 100125, China

1 The authors contribute equally to this article.

Peer review under responsibility of Tsinghua University Press.

Keywords:
Transcriptomic analysis, Liver injury, Antioxidants, Condensed tannins, High cholesterol
Cite this article:
Li X, Pu Y, Liu B, et al. Biochemistry and transcriptome analysis reveal condensed tannins alleviate liver injury induced by regulating cholesterol metabolism pathway. Food Science and Human Wellness, 2024, 13(2): 909-919. https://doi.org/10.26599/FSHW.2022.9250078
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Abstract Full text Electronic supplementary material About this article

Free cholesterol has been considered to be a critical risk factor of nonalcoholic fatty liver disease (NAFLD). It remains unknown whether dietary intake of condensed tannins (CTs) have distinguishable effects to alleviate liver damage caused by a high cholesterol diet. Male C57BL/6 mice were fed a high cholesterol diet for 6 weeks, and given CTs treatment at a dosage of 200 mg/(kg·day) at the same time. The results indicated that compared with mice fed a normal diet, a high cholesterol diet group resulted in significant weight loss, dysregulation of lipid metabolism in blood and liver, and oxidative stress in the liver, but CTs treatment dramatically reversed these negative effects. Hematoxylin and eosin (H&E) staining and frozen section observation manifested that CTs treatment could effectively reduce the deposition of liver cholesterol and tissue necrosis caused by high cholesterol intake. CTs alleviated liver injury mainly by regulating the expression of related genes in cholesterol metabolism pathway and AMPK phosphorylation. Our results confirmed that CTs have remarkable cholesterol lowering and anti-liver injury effects in vivo.


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About this article

Biochemistry and transcriptome analysis reveal condensed tannins alleviate liver injury induced by regulating cholesterol metabolism pathway

Show Author's information Xiangxin Lia,1, Yijing Pua,b,1, Bangdi Liuc, Xiaoming Fanga, Wenjun Penga, Weibo Jiangb( ), Wenli Tiana,( )
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
Academy of Agricultural Planning and Engineering, Ministry of Agriculture and Rural Affairs, Beijing 100125, China

1 The authors contribute equally to this article.

Peer review under responsibility of Tsinghua University Press.

Abstract

Free cholesterol has been considered to be a critical risk factor of nonalcoholic fatty liver disease (NAFLD). It remains unknown whether dietary intake of condensed tannins (CTs) have distinguishable effects to alleviate liver damage caused by a high cholesterol diet. Male C57BL/6 mice were fed a high cholesterol diet for 6 weeks, and given CTs treatment at a dosage of 200 mg/(kg·day) at the same time. The results indicated that compared with mice fed a normal diet, a high cholesterol diet group resulted in significant weight loss, dysregulation of lipid metabolism in blood and liver, and oxidative stress in the liver, but CTs treatment dramatically reversed these negative effects. Hematoxylin and eosin (H&E) staining and frozen section observation manifested that CTs treatment could effectively reduce the deposition of liver cholesterol and tissue necrosis caused by high cholesterol intake. CTs alleviated liver injury mainly by regulating the expression of related genes in cholesterol metabolism pathway and AMPK phosphorylation. Our results confirmed that CTs have remarkable cholesterol lowering and anti-liver injury effects in vivo.

Keywords: Transcriptomic analysis, Liver injury, Antioxidants, Condensed tannins, High cholesterol

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Received: 16 July 2022
Revised: 14 August 2022
Accepted: 11 September 2022
Published: 25 September 2023
Issue date: March 2024

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© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

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Acknowledgements

This work was supported by the National Basic Research Program of China (2013CB127106).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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