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Edible plant derived exosome-like nanoparticles (ELNs) have been shown to have multiple nutraceutical functions. However, the diversity of plant materials makes the plant derived ELN study challenging. More efforts are still needed to explore the feasible isolation methods of edible plant derived ELNs and the possible roles of food-derived ELNs in improving human health. In this study, a size exclusion chromatography based method was compared with the traditional ultracentrifugation method to isolate blueberry derived ELNs (B-ELNs), and the miRNA profile of B-ELNs was analyzed by high-throughput sequencing. A total of 36 miRNAs were found to be enriched in B-ELNs compared with berry tissue, and their potential cross-kingdom human gene targets were further predicted. Results showed that size exclusion chromatography was effective for B-ELN isolation. The most abundant miRNAs in B-ELNs mainly belonged to the miR166 family and miR396 family. Target gene prediction indicated that B-ELNs could potentially regulate pathways related to the human digestive system, immune system and infectious diseases.


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Characterization of blueberry exosome-like nanoparticles and miRNAs with potential cross-kingdom human gene targets

Show Author's information Yangfan LengaLiubin YangaSiyi Pana,bLeilei ZhancFang Yuana,b,( )
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Hubei Key Laboratory of Fruit & Vegetable Processing & Quality Control, Huazhong Agricultural University, Wuhan 430070, China
Wuhan GeneCreate Biological Engineering Co., Ltd., Wuhan 430070, China

Peer review under responsibility of Tsinghua University Press.

Highlights

• Size exclusion chromatography based method was effective to extract blueberry derived exosome-like nanoparticles

• Size exclusion chromatography was able to achieve higher B-ELN purity and a more complete vesicle structure compared with ultracentrifugation

• The miRNAs enriched in the B-ELNs possessed potential cross-kingdom human gene targets

Abstract

Edible plant derived exosome-like nanoparticles (ELNs) have been shown to have multiple nutraceutical functions. However, the diversity of plant materials makes the plant derived ELN study challenging. More efforts are still needed to explore the feasible isolation methods of edible plant derived ELNs and the possible roles of food-derived ELNs in improving human health. In this study, a size exclusion chromatography based method was compared with the traditional ultracentrifugation method to isolate blueberry derived ELNs (B-ELNs), and the miRNA profile of B-ELNs was analyzed by high-throughput sequencing. A total of 36 miRNAs were found to be enriched in B-ELNs compared with berry tissue, and their potential cross-kingdom human gene targets were further predicted. Results showed that size exclusion chromatography was effective for B-ELN isolation. The most abundant miRNAs in B-ELNs mainly belonged to the miR166 family and miR396 family. Target gene prediction indicated that B-ELNs could potentially regulate pathways related to the human digestive system, immune system and infectious diseases.

Keywords: Size exclusion chromatography, miRNA, Blueberry, Edible plant derived exosomes-like nanoparticles, Target gene prediction

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Publication history

Received: 05 July 2022
Revised: 04 August 2022
Accepted: 25 September 2022
Published: 25 September 2023
Issue date: March 2024

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© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

Acknowledgements

Acknowledgement

This work was supported by the National Natural Science Foundation of China (31701561).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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