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Food Science and Human Wellness 2024, 13(2): 698-708 https://doi.org/10.26599/FSHW.2022.9250059
Open Access | Issue | Published: 25 September 2023

Lactobacillus plantarum AR495 improves stress-induced irritable bowel syndrome in rats by targeting gut microbiota and Mast cell-PAR2-TRPV1 signaling pathway

Show Author's Information Hongyun Zhanga,,  Guangqiang Wanga,  Zhiqiang Xionga,  Zhuan Liaob,  Yangyan Qianb,  Xin Songa,  Li Suia,  Lianzhong Aib,  Yongjun Xiaa( )
Shanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
Department of Gastroenterol, Digestive Endoscopy Center, Shanghai Hospital, Shanghai 200093, China

Peer review under responsibility of Tsinghua University Press.

Keywords:
Microbiota, Dorsal root ganglia, Lactobacillus plantarum, Irritable bowel syndrome, Visceral sensitivity, Tryptase
Cite this article:
Zhang H, Wang G, Xiong Z, et al. Lactobacillus plantarum AR495 improves stress-induced irritable bowel syndrome in rats by targeting gut microbiota and Mast cell-PAR2-TRPV1 signaling pathway. Food Science and Human Wellness, 2024, 13(2): 698-708. https://doi.org/10.26599/FSHW.2022.9250059
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Abstract Full text About this article

Probiotics have great potential in regulating intestinal pain. In this study, the effects of Lactobacillus plantarum AR495 on the visceral sensitivity and gut microbiota of irritable bowel syndrome (IBS) rats were studied. The results showed that tryptase released after mast cell activation and degranulation plays a key role in visceral pain, and L. plantarum AR495 reduced the stimulation of colonic mast cells and the expression of protease-activated receptor 2 (PAR2) and TRPV1 in dorsal root ganglia. Research further showed that supplementation with L. plantarum AR495 increased the level of short-chain fatty acids (SCFAs) and enhanced the barrier function of the colo n. In addition, the microbiota analysis of the colon indicated that L. plantarum AR495 promoted the proliferation of Bifidobacterium and inhibited the proliferation of Lachnospiraceae, which alleviated the imbalance of the intestinal microbiota caused by IBS to a certain extent. In total, L. plantarum AR495 might reduce visceral sensitivity through the Mast cell-PAR2-TRPV1 signaling pathway by maintaining the homeostasis of the intestinal barrier.


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About this article

Lactobacillus plantarum AR495 improves stress-induced irritable bowel syndrome in rats by targeting gut microbiota and Mast cell-PAR2-TRPV1 signaling pathway

Show Author's information Hongyun Zhanga,, Guangqiang Wanga, Zhiqiang Xionga, Zhuan Liaob, Yangyan Qianb, Xin Songa, Li Suia, Lianzhong Aib, Yongjun Xiaa( )
Shanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
Department of Gastroenterol, Digestive Endoscopy Center, Shanghai Hospital, Shanghai 200093, China

Peer review under responsibility of Tsinghua University Press.

Highlights

1. L. plantarum AR495 decreased the visceral sensitivity of the IBS group

2. L. plantarum AR495 restrained the degranulation of mast cells in the colon

3. L. plantarum AR495 prevented the destruction of colon barrier function

4. L. plantarum AR495 increased the SCFAs and the abundance of Bifidobacterium

5. L. plantarum AR495 inhibited the mast cells-tryptase-PAR2-TRPV1 pathway

Abstract

Probiotics have great potential in regulating intestinal pain. In this study, the effects of Lactobacillus plantarum AR495 on the visceral sensitivity and gut microbiota of irritable bowel syndrome (IBS) rats were studied. The results showed that tryptase released after mast cell activation and degranulation plays a key role in visceral pain, and L. plantarum AR495 reduced the stimulation of colonic mast cells and the expression of protease-activated receptor 2 (PAR2) and TRPV1 in dorsal root ganglia. Research further showed that supplementation with L. plantarum AR495 increased the level of short-chain fatty acids (SCFAs) and enhanced the barrier function of the colo n. In addition, the microbiota analysis of the colon indicated that L. plantarum AR495 promoted the proliferation of Bifidobacterium and inhibited the proliferation of Lachnospiraceae, which alleviated the imbalance of the intestinal microbiota caused by IBS to a certain extent. In total, L. plantarum AR495 might reduce visceral sensitivity through the Mast cell-PAR2-TRPV1 signaling pathway by maintaining the homeostasis of the intestinal barrier.

Keywords: Microbiota, Dorsal root ganglia, Lactobacillus plantarum, Irritable bowel syndrome, Visceral sensitivity, Tryptase

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Publication history

Received: 06 March 2022
Revised: 11 July 2022
Accepted: 15 September 2022
Published: 25 September 2023
Issue date: March 2024

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© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

Acknowledgements

Acknowledgment

This work was supported by the shanghai agriculture applied technology development program (2019-02-08-00-07-F01152); the national science fund for distinguished young scholars (32025029); the shanghai engineering research center of food microbiology program (19DZ2281100); and the national key R&D program of china (2018YFC1604305).

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