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Food Science and Human Wellness 2024, 13(2): 611-620 https://doi.org/10.26599/FSHW.2022.9250052
Open Access | Issue | Published: 25 September 2023

Demonstration of safety characteristics and effects on gut microbiota of Lactobacillus gasseri HMV18

Show Author's Information Xianxian Jiaa,b,1,  Miaomiao Jiab,c,1,  Xiang Gaoa,b,1,  Xiang Lic,  Mengyuan Wangc,  Shengqiang Duc,  Rui Huangc,  Xiaotong Lic,  Jun Zhangc,  Shujin Lib,d,  Chunling Mab,d,  Yan Zhangb,e( ),  Bin Congb,d,( )
Department of Pathogen Biology, Institute of basic Medicine, Hebei Medical University, Shijiazhuang 050017, China
Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology, Chinese Academy of Medical Sciences, Shijiazhuang 050017, China
Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China
College of Forensic Medicine, Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Hebei Medical University, Shijiazhuang 050017, China
Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang 050227, China

1 These authors contributed equally to this work.

Peer review under responsibility of Tsinghua University Press.

Keywords:
Gut microbiota, Probiotics, Safety assessment, Lactobacillus gasseri
Cite this article:
Jia X, Jia M, Gao X, et al. Demonstration of safety characteristics and effects on gut microbiota of Lactobacillus gasseri HMV18. Food Science and Human Wellness, 2024, 13(2): 611-620. https://doi.org/10.26599/FSHW.2022.9250052
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Abstract Full text Electronic supplementary material About this article

Human normal flora is a source of probiotics. The safety characteristics of a specific isolate determine its application in foods or drugs. The food-borne-pathogen antagonist strain Lactobacillus gasseri HMV18 is one of the isolates from normal human f lora. In this work, we assessed the in vitro pH tolerance, bile tolerance, biogenic amine production, mucin utilization, and safety of in vivo administration to mice to evaluate general health, organ-body weight index, organ histopathological change, whether L. gasseri HMV18 can colonize in the gut or modulate the gut microbiota after oral administration. The results suggest that L. gasseri HMV18 can tolerate pH 3 for 2 h, 3% bile for 3 h, biogenic amine negative, mucin usage negative, does not encode verif ied toxins, and cause no visible change in mice’s organs. L. gasseri HMV18 might not colonize in mice’s gut, but can signif icantly affect the structure of gut microbiota. A bibliographical survey suggested that there were as few as 8 opportunistic infection cases from 1984 to 2022 and that the possibility for L. gasseri to cause infection is relatively low. Therefore, this work provides a basis for the foods or drugs application of L. gasseri HMV18 and gives a map of experiments for the safety assessment of probiotics.


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Abstract
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Electronic supplementary material
About this article

Demonstration of safety characteristics and effects on gut microbiota of Lactobacillus gasseri HMV18

Show Author's information Xianxian Jiaa,b,1, Miaomiao Jiab,c,1, Xiang Gaoa,b,1, Xiang Lic, Mengyuan Wangc, Shengqiang Duc, Rui Huangc, Xiaotong Lic, Jun Zhangc, Shujin Lib,d, Chunling Mab,d, Yan Zhangb,e( ), Bin Congb,d,( )
Department of Pathogen Biology, Institute of basic Medicine, Hebei Medical University, Shijiazhuang 050017, China
Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology, Chinese Academy of Medical Sciences, Shijiazhuang 050017, China
Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China
College of Forensic Medicine, Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Hebei Medical University, Shijiazhuang 050017, China
Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang 050227, China

1 These authors contributed equally to this work.

Peer review under responsibility of Tsinghua University Press.

Highlights

1. Lactobacillus gasseri HMV18 is a potential probiotic.

2. Bioamine, mucin utilization and genome encoded mammalian toxin are negative.

3. Mice orally administrated HMV18 do no observable harm to organs but can affect the structure and composition of gut microbiota.

4. We offered a route map for design safety evaluation experiments.

Abstract

Human normal flora is a source of probiotics. The safety characteristics of a specific isolate determine its application in foods or drugs. The food-borne-pathogen antagonist strain Lactobacillus gasseri HMV18 is one of the isolates from normal human f lora. In this work, we assessed the in vitro pH tolerance, bile tolerance, biogenic amine production, mucin utilization, and safety of in vivo administration to mice to evaluate general health, organ-body weight index, organ histopathological change, whether L. gasseri HMV18 can colonize in the gut or modulate the gut microbiota after oral administration. The results suggest that L. gasseri HMV18 can tolerate pH 3 for 2 h, 3% bile for 3 h, biogenic amine negative, mucin usage negative, does not encode verif ied toxins, and cause no visible change in mice’s organs. L. gasseri HMV18 might not colonize in mice’s gut, but can signif icantly affect the structure of gut microbiota. A bibliographical survey suggested that there were as few as 8 opportunistic infection cases from 1984 to 2022 and that the possibility for L. gasseri to cause infection is relatively low. Therefore, this work provides a basis for the foods or drugs application of L. gasseri HMV18 and gives a map of experiments for the safety assessment of probiotics.

Keywords: Gut microbiota, Probiotics, Safety assessment, Lactobacillus gasseri

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Publication history

Received: 13 February 2023
Revised: 26 February 2023
Accepted: 04 March 2023
Published: 25 September 2023
Issue date: March 2024

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© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

Acknowledgements

Acknowledgement

This work was financially supported by postdoctoral funding of Hebei Medical University, Hebei Province Postdoctoral Research Project Funding (B2022003035), Natural Science Foundation of Hebei Province (H2020206579), CAMS Innovation Found for Medical Sciences (2019-I2M-5-055), 2023 Scientific Research Projects of Colleges and Universities in Hebei Province (QN2023131), S & T Program of Hebei (18277743D), Undergraduate Innovation Experiment Project from Hebei Medical University (USIP2019008), and Spring rain project of Hebei Medical University (CYCZ201906).

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