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Food Science and Human Wellness 2024, 13(1): 401-413 https://doi.org/10.26599/FSHW.2022.9250034
Research Article | Open Access | Issue | Published: 01 June 2023

Protective mechanism of Coprinus comatus polysaccharide on acute alcoholic liver injury in mice, the metabolomics and gut microbiota investigation

Show Author's Information Jinyan Yua,  Jianguang Sunb,  Min Suna,  Weidong Lia,  Dongmei Qic,  Yongqing Zhanga,  Chunchao Hana,( )
School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
Hepatology Department of Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250355, China
Experimental center, Shandong University of Traditional Chinese Medicine, Jinan 250355, China

Peer review under responsibility of Tsinghua University Press.

Keywords:
Gut microbiota, Polysaccharide, Metabolomics, Alcoholic liver disease, Coprinus comatus
Cite this article:
Yu J, Sun J, Sun M, et al. Protective mechanism of Coprinus comatus polysaccharide on acute alcoholic liver injury in mice, the metabolomics and gut microbiota investigation. Food Science and Human Wellness, 2024, 13(1): 401-413. https://doi.org/10.26599/FSHW.2022.9250034
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Abstract Full text About this article

Coprinus comatus polysaccharide (CCP) has significant hepatoprotective effect. To explore hepatoprotective mechanism of CCP, the study analyzed preventive effect of CCP on acute alcoholic liver injury in mice by histopathological examination and biochemical analysis. Simultaneously, hepatoprotective mechanism was also analyzed in conjunction with metabolomics and proliferation of gut microbiota. The results showed that CCP significantly decreased alanine aminotransferase (ALT), aspartate aminotransferase (AST) and triglyceride (TG) levels in serum of alcoholic liver disease (ALD) mice. Histopathological examination showed that CCP can significantly improve liver damage. Metabolomics results showed that there were significant differences in the level of metabolites in liver tissue of control group, ALD group and CCP group, including taurine, xanthosine, fumaric acid and arachidonic acid, among others. Metabolites pathways analysis showed that hepatoprotective effect of CCP was related to energy metabolism, biosynthesis of unsaturated fatty acids, amino acids metabolism and lipid metabolism. Additionally, CCP inhibited an increase in the number of Clostridium perfringens, Enterobacteriaceae and Enterococcus, and a decrease in the number of Lactobacillus and Bifidobacterium in the gut of ALD mice. All these findings suggested that CCP treatment reversed the phenotype of ethanol-induced liver injury and the associated metabolites pathways.


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About this article

Protective mechanism of Coprinus comatus polysaccharide on acute alcoholic liver injury in mice, the metabolomics and gut microbiota investigation

Show Author's information Jinyan Yua, Jianguang Sunb, Min Suna, Weidong Lia, Dongmei Qic, Yongqing Zhanga, Chunchao Hana,( )
School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
Hepatology Department of Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250355, China
Experimental center, Shandong University of Traditional Chinese Medicine, Jinan 250355, China

Peer review under responsibility of Tsinghua University Press.

Abstract

Coprinus comatus polysaccharide (CCP) has significant hepatoprotective effect. To explore hepatoprotective mechanism of CCP, the study analyzed preventive effect of CCP on acute alcoholic liver injury in mice by histopathological examination and biochemical analysis. Simultaneously, hepatoprotective mechanism was also analyzed in conjunction with metabolomics and proliferation of gut microbiota. The results showed that CCP significantly decreased alanine aminotransferase (ALT), aspartate aminotransferase (AST) and triglyceride (TG) levels in serum of alcoholic liver disease (ALD) mice. Histopathological examination showed that CCP can significantly improve liver damage. Metabolomics results showed that there were significant differences in the level of metabolites in liver tissue of control group, ALD group and CCP group, including taurine, xanthosine, fumaric acid and arachidonic acid, among others. Metabolites pathways analysis showed that hepatoprotective effect of CCP was related to energy metabolism, biosynthesis of unsaturated fatty acids, amino acids metabolism and lipid metabolism. Additionally, CCP inhibited an increase in the number of Clostridium perfringens, Enterobacteriaceae and Enterococcus, and a decrease in the number of Lactobacillus and Bifidobacterium in the gut of ALD mice. All these findings suggested that CCP treatment reversed the phenotype of ethanol-induced liver injury and the associated metabolites pathways.

Keywords: Gut microbiota, Polysaccharide, Metabolomics, Alcoholic liver disease, Coprinus comatus

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Publication history

Received: 14 April 2022
Revised: 17 May 2022
Accepted: 08 June 2022
Published: 01 June 2023
Issue date: January 2024

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© 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press.

Acknowledgements

The current project is funded by Shandong Provincial Natural Science Foundation, China (ZR2020MH370), Major Science and Technology Innovation in Shandong Province (2017CXGC1307) and Ji'nan Science and Technology Project (201303055).

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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