Determination of 24 tranquilizer drugs and 27 anesthetic drugs in livestock meat by QuEChERS combined with ultra-high performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry

This study established a method for detecting 24 tranquilizer drugs and 27 anesthetic drugs in livestock meat by combining the QuEChERS method with ultra-high performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry (UPLC-Q-Orbitrap HRMS). The results demonstrated that the 51 target compounds exhibited good linear relationships within their respective ranges (R² ≥ 0.995 2), low detection and quantification limits, satisfactory spike recovery rates (84.0%–111.9%), and good precision (relative standard deviation: 2.5%–8.5%), meeting the requirements for quantitative analysis. This approach combines the convenience and speed of QuEChERS with the high accuracy of UPLC-Q-Orbitrap HRMS, enabling efficient qualitative confirmation and reliable quantitative analysis of 51 target compounds. With its rapid, accurate, and straightforward advantages, this method holds significant potential for application in food safety monitoring of livestock meat, providing technical support for regulating the illegal use of sedatives and anesthetics in animal-derived foods.