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This paper investigated the changes in the texture, flavor, nutrition, and digestive characteristics of soft-boiled and marinated eggs (SME) during pressurized heat treatment (PHT) at 121 ℃ and 125 kPa for 15, 30, and 45 min. Eggs were pre-cooked in boiling water for 5 min, cooled and peeled in cool water, and then immersed in the brine at 65 ℃ for 2 h to prepare marinated eggs. The results showed that the hardness of SME (121 ℃, 0 min) was the lowest, with the egg white and yolk hardness were 1 368.05 and 530.43 g, respectively. After 15 min of PHT, the hardness of egg white and yolk increased significantly to 2 077.54 and 1 492.75 g (P < 0.05). The relaxation time of non-fluidizable water and free water of egg yolk decreased after PHT. The electronic nose test clearly distinguished the flavor difference before and after PHT, and moderate lipid oxidation was helpful to form new flavors. The protein digestibility of the SME yolk was significantly higher than the PHT groups. Vitamin A content of the SME (0.24 mg/100 g) was 1.26 times higher than the 30 min PHT group. After 30 min heat treatment, the vitamin E content of egg yolk decreased by 7.88%, phosphatidylcholine (PC) and phosphatidylethanolamine (PE) decreased significantly from 2.02 and 1.52 g/100 g to 1.73 and 0.92 g/100 g, respectively. Low temperature group (SME) maintained higher levels of fat-soluble vitamins and lecithin in the egg yolk than PHT groups.


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The effect of pressurized heat treatment on the textural, flavor, nutritional, and digestive characteristics of soft-boiled and marinated eggs

Show Author's information Hairu Zhang1Zuyue Li1Dong Uk Ahn2Xi Huang1( )
Key Laboratory of Environment Correlative Dietology, Ministry of Education, National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Animal Science Department, Iowa State University, Ames, USA

Abstract

This paper investigated the changes in the texture, flavor, nutrition, and digestive characteristics of soft-boiled and marinated eggs (SME) during pressurized heat treatment (PHT) at 121 ℃ and 125 kPa for 15, 30, and 45 min. Eggs were pre-cooked in boiling water for 5 min, cooled and peeled in cool water, and then immersed in the brine at 65 ℃ for 2 h to prepare marinated eggs. The results showed that the hardness of SME (121 ℃, 0 min) was the lowest, with the egg white and yolk hardness were 1 368.05 and 530.43 g, respectively. After 15 min of PHT, the hardness of egg white and yolk increased significantly to 2 077.54 and 1 492.75 g (P < 0.05). The relaxation time of non-fluidizable water and free water of egg yolk decreased after PHT. The electronic nose test clearly distinguished the flavor difference before and after PHT, and moderate lipid oxidation was helpful to form new flavors. The protein digestibility of the SME yolk was significantly higher than the PHT groups. Vitamin A content of the SME (0.24 mg/100 g) was 1.26 times higher than the 30 min PHT group. After 30 min heat treatment, the vitamin E content of egg yolk decreased by 7.88%, phosphatidylcholine (PC) and phosphatidylethanolamine (PE) decreased significantly from 2.02 and 1.52 g/100 g to 1.73 and 0.92 g/100 g, respectively. Low temperature group (SME) maintained higher levels of fat-soluble vitamins and lecithin in the egg yolk than PHT groups.

Keywords: texture, flavor, marinated egg, pressurized heat treatment, protein digestibility

References(52)

[1]

J. T. Wang, Y. J. Chi, Y. Cheng, et al., Physicochemical properties, in vitro digestibility and antioxidant activity of dry-heated egg white protein, Food Chem. 246 (2018) 18–25. https://doi.org/10.1016/j.foodchem.2017.10.128.

[2]

Y. Y. Shao, Y. Zhao, M. S. Xu, et al., Effects of copper ions on the characteristics of egg white gel induced by strong alkali, Poultry Sci. 96 (2017) 4116–4123. https://doi.org/10.3382/ps/pex213.

[3]

S. A. Woodward, Texture and microstructure of heat-formed egg white, egg yolk and whole egg gels, Dissertation Abstracts International B 45 (1985) 2823.

[4]

M. X. Zhang, X. S. Hu, S. Li, et al., Hepatoprotective effects of ethyl pyruvate against CCl4-induced hepatic fibrosis via inhibition of TLR4/NF-κB signaling and up-regulation of MMPs/TIMPs ratio, Clin. Res. Hepatol. Gas. 42 (2018) 72–81. https://doi.org/10.1016/j.clinre.2017.04.008.

[5]

S. Q. Gu, W. L. Dai, Y. Q. Chong, et al., The binding of key fishy off-flavor compounds to silver carp proteins: a thermodynamic analysis, RSC Adv. 10 (2020) 11292–11299. https://doi.org/10.1039/d0ra01365j.

[6]

L. K. Mao, Y. H. Roos, S. Miao, Study on the rheological properties and volatile release of cold-set emulsion-filled protein gels, J. Agr. Food Chem. 62 (2014) 11420–11428. https://doi.org/10.1021/jf503931y.

[7]

L. L. Zhang, F. X. Zhang, X. Wang, Changes of protein secondary structures of pollock surimi gels under high-temperature (100 ℃ and 120 ℃) treatment, J. Food Eng. 171 (2016) 159–163. https://doi.org/10.1016/j.jfoodeng.2015.10.025.

[8]

H. Zhang, Y. Z. Zhu, S. Chen, et al., Determination of the effects of different high-temperature treatments on texture and aroma characteristics in Alaska pollock surimi, Food Sci. Nutr. 6 (2018) 2079–2091. https://doi.org/10.1002/fsn3.763.

[9]

K. C. Cheng, S. H. Chen, A. I. Yeh, Physicochemical properties and in vitro digestibility of rice after parboiling with heat moisture treatment, J. Cereal Sci. 85 (2019) 98–104. https://doi.org/10.1016/j.jcs.2018.11.016.

[10]

C. Fontes-Candia, P. Jiménez-Barrios, B. Miralles, et al., Development of polysaccharide-casein gel-like structures resistant to in vitro gastric digestion, Food Hydrocoll. 127 (2022) 107505. https://doi.org/10.1016/j.foodhyd.2022.107505.

[11]

M. Li, J. Wang, F. F. Wang, et al., Insights into the multi-scale structure of wheat starch following acylation: physicochemical properties and digestion characteristics, Food Hydrocoll. 124 (2022) 107347. https://doi.org/10.1016/j.foodhyd.2021.107347.

[12]

C. B. Zhao, Q. Li, N. N. Hu, et al., Improvement of structural characteristics and in vitro digestion properties of zein by controlling postharvest ripening process of corn, Food Cont. 142 (2022) 109221. https://doi.org/10.1016/j.foodcont.2022.109221.

[13]

T. Farjami, J. Babaei, F. Nau, et al., Effects of thermal, non-thermal and emulsification processes on the gastrointestinal digestibility of egg white proteins, Trends Food Sci. Tech. 107 (2021) 45–56. https://doi.org/10.1016/j.jpgs.2020.11.029.

[14]

V. Lechevalier, C. Guérin-Dubiard, M. Anton, et al., Effect of dry heat treatment of egg white powder on its functional, nutritional and allergenic properties, J. Food Eng. 195 (2017) 40–51. https://doi.org/10.1016/j.jfoodeng.2016.09.022.

[15]

X. F. Wang, N. Qiu, Y. P. Liu, Effect of different heat treatments on in vitro digestion of egg white proteins and identification of bioactive peptides in digested products, J. Food Sci. 83 (2018) 1140–1148. https://doi.org/10.1111/1750-3841.14107.

[16]

H. Xue, Y. G. Tu, M. Xu, et al., Changes in physicochemical properties, gel structure and in vitro digestion of marinated egg white gel during braising, Food Chem. 330 (2020) 127321. https://doi.org/10.1016/j.foodchem.2020.127321.

[17]

D. Y. Tang, R. Wang, X. H. He, et al., Comparison of the edible quality of liquid egg with different cooking methods and their antioxidant activity after in vitro digestion, Food Res. Int. 140 (2021) 110013. https://doi.org/10.1016/j.foodres.2020.110013.

[18]

J. H. Shao, Y. M. Deng, L. Song, et al., Investigation the effects of protein hydration states on the mobility water and fat in meat batters by LF-NMR technique, LWT-Food Sci. Tech. 66 (2016) 1–6. https://doi.org/10.1016/j.lwt.2015.10.008.

[19]

Z. J. Bao, D. Kang, C. Li, et al., Effect of salting on the water migration, physicochemical and textural characteristics, and microstructure of quail eggs, LWT-Food Sci. Tech. 132 (2020) 109847. https://doi.org/10.1016/j.lwt.2020.109847.

[20]

C. H. Wu, C. H. Yuan, S. G. Chen, et al., The effect of curdlan on the rheological properties of restructured ribbonfish (Trichiurus spp.) meat gel, Food Chem. 179 (2015) 222–231. https://doi.org/10.1016/j.foodchem.2015.01.125.

[21]

J. Shi, Y. G. Nian, D. D. Da, et al., Characterization of flavor volatile compounds in sauce spareribs by gas chromatography-mass spectrometry and electronic nose, LWT-Food Sci. Tech. 124 (2020) 109182. https://doi.org/10.1016/j.lwt.2020.109182.

[22]

N. Marušić, M. Petrović, S. Vidaček, et al., Characterization of traditional Istrian dry-cured ham by means of physical and chemical analyses and volatile compounds, Meat Sci. 88 (2011) 786–790. https://doi.org/10.1016/j.meatsci.2011.02.033.

[23]
A. Brodkorb, L. Egger, M. Alminger, et al. , INFOGEST static in vitro simulation of gastrointestinal food digestion, Nat. Protoc. 14 (2019) 991–1014. https://doi.org/10.1038/s41596-018-0119-1.
DOI
[24]

A. Camara, M. V. Geraldi, P. K. Okuro, et al., Satiety and in vitro digestibility of low saturated fat Bologna sausages added of chia mucilage powder and chia mucilage-based emulsion gel, J. Funct. Foods 65 (2020) 103753. https://doi.org/10.1016/j.jff.2019.103753.

[25]
L. B. Pham, B. Wang, B. Zisu, et al. , In vitro digestion of flaxseed oil encapsulated in phenolic compound adducted flaxseed protein isolate-flaxseed gum complex coacervates, Food Hydrocoll. 112 (2021) 106325. https://doi.org/10.1016/j.foodhyd.2020.106325.
DOI
[26]

U. K. Laemmli, Cleavage of structural proteins during assembly of head of bacteriophage-T4, Nature 227 (1970) 680. https://doi.org/10.1038/227680a0.

[27]
F. Mohammadsadeghi, M. Afsharmanesh, M. Salarmoini, et al., The effect of replacing sodium selenite with selenium-chitosan in laying hens on production performance, egg quality, egg selenium concentration, microbial population, immunological response, antioxidant enzymes, and fatty acid composition, Poultry Sci. (2023) 102983. https://doi.org/10.1016/j.psj.2023.102983.
DOI
[28]

T. Tsuji, T. Yuri, T. Terada, et al., Application of enzymatic fluorometric assays to quantify phosphatidylcholine, phosphatidylethanolamine and sphingomyelin in human plasma lipoproteins, Chem. Phys. Lipids. 238 (2021) 105102. https://doi.org/10.1016/j.chemphyslip.2021.105102.

[29]

S. S. Cheng, T. Zhang, X. H. Wang, et al., Influence of salting processes on water and lipid dynamics, physicochemical and microstructure of duck egg, LWT-Food Sci. Tech. 95 (2018) 143–149. https://doi.org/10.1016/j.lwt.2018.04.074.

[30]

S. G. Li, K. P. Wang, Q. Huang, et al., Microwave pretreatment enhanced the properties of ovalbumin-inulin-oil emulsion gels and improved the storage stability of pomegranate seed oil, Food Hydrocoll. 113 (2021) 106548. https://doi.org/10.1016/j.foodhyd.2020.106548.

[31]

L. L. Xu, Y. Q. Lv, Y. J. Su, et al., Enhancing gelling properties of high internal phase emulsion-filled chicken gels: effect of droplet fractions and salts, Food Chem. 367 (2022) 130663. https://doi.org/10.1016/j.foodchem.2021.130663.

[32]

H. Xue, G. W. Zhang, T. F. Han, et al., Improvement of gel properties and digestibility of the water-soluble polymer of tea polyphenol-egg white under thermal treatment, Food Chem. 372 (2022) 131319. https://doi.org/10.1016/j.foodchem.2021.131319.

[33]

Q. Li, X. Y. Sun, E. Mubango, et al., Effects of protein and lipid oxidation on the water holding capacity of different parts of bighead carp: eye, dorsal, belly and tail muscles, Food Chem. 423 (2023) 136238. https://doi.org/10.1016/j.foodchem.2023.136238.

[34]

H. Shen, J. S. Elmore, M. M. Zhao, et al., Effect of oxidation on the gel properties of porcine myofibrillar proteins and their binding abilities with selected flavour compounds, Food Chem. 329 (2020) 127032. https://doi.org/10.1016/j.foodchem.2020.127032.

[35]

L. K. Mao, S. Miao, F. Yuan, et al., Study on the textural and volatile characteristics of emulsion filled protein gels as influenced by different fat substitutes, Food Res. Int. 103 (2018) 1–7. https://doi.org/10.1016/j.foodres.2017.10.024.

[36]

X. L. Xiang, G. Hu, Z. S. Yu, et al., Changes in the textural and flavor characteristics of egg white emulsion gels induced by lipid and thermal treatment, Innov. Food Sci. Emerg. 79 (2022) 103054. https://doi.org/10.1016/j.ifset.2022.103054.

[37]

G. Han, Y. H. Fan, Q. Chen, et al., Quality and flavor changes in beef jerky caused by high hydrostatic pressure combined with moisture regulator treatments during storage, Food Sci. Anim. Produ. 1(1) (2023) 9240001. https://doi.org/10.26599/FSAP.2023.9240001.

[38]

S. Corral, A. Salvador, M. Flores, Salt reduction in slow fermented sausages affects the generation of aroma active compounds, Meat Sci. 93(3) (2013) 776–785. https://doi.org/10.1016/j.meatsci.2012.11.040.

[39]

T. Perez-Palacios, J. Ruiz-Carrascal, E. Jimenez-Martin, et al., Improving the lipid profile of ready-to-cook meat products by addition of omega-3 microcapsules: effect on oxidation and sensory analysis, J. Sci. Food Agr. 98 (2018) 5302–5312. https://doi.org/10.1002/jsfa.9069.

[40]

Y. J. Lee, W. B. Yoon, Effects of particle size and heating time on thiobarbituric acid (TBA) test of soybean powder, Food Chem. 138 (2013) 841–850. https://doi.org/10.1016/j.foodchem.2012.11.113.

[41]

Y. F. Yang, M. N. Luo, Q. H. Hu, et al., Comparative analysis of three accelerated lipid oxidation methods, Food Sci. 43 (2022) 337–346. https://doi.org/10.7506/spkx1002-6630-20210121-233.

[42]

Z. G. Ke, Y. Bai, Y. W. Bai, et al., Cold plasma treated air improves the characteristic flavor of dry-cured black carp through facilitating lipid oxidation, Food Chem. 377 (2022) 131932. https://doi.org/10.1016/j.foodchem.2021.131932.

[43]

M. M. Campo, G. R. Nute, S. I. Hughes, et al., Flavour perception of oxidation in beef, Meat Sci. 72 (2006) 303–311. https://doi.org/10.1016/j.meatsci.2005.07.015.

[44]
A. Asensio-Grau, J. Calvo-Lerma, A. Heredia, et al., In vitro digestion of salmon: influence of processing and intestinal conditions on macronutrients digestibility, Food Chem. 342 (2021) 128387. https://doi.org/10.1016/j.foodchem.2020.128387.
DOI
[45]

L. Qi, R. M. Boom, A. E. M. Janssen, Digestion of protein and protein gels in simulated gastric environment, LWT-Food Sci. Tech. 63 (2015) 161–168. https://doi.org/10.1016/j.lwt.2015.03.087.

[46]

Y. J. Ning, B. Cui, C. Yuan, Decreasing the digestibility of debranched corn starch by encapsulation with konjac glucomannan, Food Hydrocoll. 107 (2020) 105966. https://doi.org/10.1016/j.foodhyd.2020.105966.

[47]

Y. Ma, Y. Liu, H. Y. Yu, et al., Biological activities and in vitro digestion characteristics of glycosylated α-lactalbumin prepared by microwave heating: impacts of ultrasonication, LWT-Food Sci. Tech. 158 (2022) 113141. https://doi.org/10.1016/j.lwt.2022.113141.

[48]
J. H. Sun, O. L. Beong, E. A. Decker, et al. , In vitro human digestion models for food applications, Food Chem. 125 (2011) 1–12. https://doi.org/10.1016/j.foodchem.2010.08.036.
DOI
[49]

K. Wattinee, C. Sanguansri, Changes in physical and gelling properties of freeze-dried egg white as a result of temperature and relative humidity, J. Sci. Food Agr. 96 (2016) 4423–4431. https://doi.org/10.1002/jsfa.7653.

[50]

Y. Yao, T. T. Liu, N. Wu, et al., An efficient, scalable and environmentally friendly separation method for ovoinhibitor from chicken egg white, LWT-Food Sci. Tech. 127 (2020) 109367. https://doi.org/10.1016/j.lwt.2020.109367.

[51]

M. Quintero-Angel, J. Martínez-Girón, D. M. Mendoza-Salazar, Traditional Colombian cuisine corn dough: characterization and evaluation of the effect of heat treatment on selected water-soluble vitamins and minerals, J. Food Compos. Anal. 116 (2023) 105054. https://doi.org/10.1016/j.jfca.2022.105054.

[52]

J. L. Fidalgo Rodríguez, P. Dynarowicz-Latka, J. Miñones Conde, How unsaturated fatty acids and plant stanols affect sterols plasma level and cellular membranes? Review on model studies involving the Langmuir monolayer technique, Chem. Phys. Lipids. 232 (2020) 104968. https://doi.org/10.1016/j.chemphyslip.2020.104968.

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Received: 22 June 2023
Revised: 12 July 2023
Accepted: 31 July 2023
Published: 14 September 2023
Issue date: October 2023

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© Beijing Academy of Food Sciences 2023.

Acknowledgements

This research was supported by the National Natural Science Foundation of China (32072237).

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Food Science of Animal Products published by Tsinghua University Press. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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