Study on the protein adsorption and its properties of self-assembled chitosan coating

The protein adsorption properties and biocompatibility of common soft materials often cannot satisfy the functional requirements in the field of cell culture and biological diagnosis. The preparation of polyelectrolyte coating on the surface of materials is an effective means to solve this problem. Due to its excellent cell affinity and bio-adhesion, chitosan has become an excellent carrier in biomedical field. Therefore, a polyelectrolyte coating of polydopamine and polyacrylic acid was prepared by the polymerization and co-deposition tactic under acidic surroundings, and the self-assembly of chitosan was carried out on the polyelectrolyte coating to investigate the factors that affect the protein adsorption performance. The study demonstrates that the chitosan coating shows outstanding wetting performances, with a 57.54% reduction in water contact angle after the modification. Furthermore, protein adsorption on the original surface is significantly improved. The surface protein adsorption capacity increases with the increasing chitosan content, and the maximum adsorption capacity is 4.43 times higher than that of the uncoated. Additionally, the chitosan coating had remarkable biocompatibility. The survival rate of mouse osteoblasts cultured on the modified was 35% bigger than that of the original, and the cell proliferation was promoted. The fabrication tactic in this study allows for the preparation of chitosan coatings on the polyelectrolyte coating of various material surfaces with controllable surface properties, and provides new insights into the preparation tactic of biological coatings for protein adsorption.