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Research Article | Open Access | Online First

Anti-virulence potential of carvone against Serratia marcescens

Jin-Wei Zhou1,2( )Kun-Yuan Yin1Wen-Qi Luo1Ao Chen1Zhe-Wen Liang1Peng-Cheng Ji1Yu-Jie Wang1Xin-Nan Wang1
School of Food and Biological Engineering, Xuzhou University of Technology, Xuzhou 221018, China
Institute of Food Microbiology, Xuzhou University of Technology, Xuzhou 221018, China
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Highlights

(1) Carvone inhibited the production of virulence factors secreted by S. marcescens

(2) Carvone enhanced the susceptibility of bacterial/biofilm cells to antibiotics

(3) Carvone inhibited the expressions of genes involved in virulence, antioxidant enzymes and biofilms

(4) Carvone enhanced oxidative stress and increased permeability of cell membrane

Graphical Abstract

Carvone treatment repressed the activities of antioxidant enzymes and resulted in the enhancement of oxidative stress. The enhanced oxidative stress improved the permeability membrane and eventually increased the susceptibility of biofilms to antibiotics.

Abstract

Serratia marcescens is a common opportunistic pathogen that causes infections in clinical patients. The aim of this study was to investigate the inhibitory efficiency of carvone on the production of virulence factors and evaluated the susceptibility of antibiotics against biofilms of S. marcescens in combination with carvone. Carvone treatment notably inhibited the production of lipase, prodigiosin, extracellular polysaccharides (EPS), and swimming and swarming motilities. Carvone exposure also reduced the formation of biofilms in S. marcescens NJ01. When exposed to antibiotics in combination with carvone, the inhibitory impact was notably improved. In addition, the expressions of genes glp and katG involved in the synthesis of glutathione peroxidase and catalase, respectively, were significantly suppressed after treatment with carvone. The suppression of antioxidant enzymes resulted in the improvement of reactive oxygen species (ROS) and H2O2, enhanced the permeability of membrane, and eventually increased the susceptibility of biofilms or planktonic cells to antibiotics. The data mentioned above indicated that carvone is expected to play an important role in the control and treatment of S. marcescns infection.

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Food & Medicine Homology
Cite this article:
Zhou J-W, Yin K-Y, Luo W-Q, et al. Anti-virulence potential of carvone against Serratia marcescens. Food & Medicine Homology, 2024, https://doi.org/10.26599/FMH.2024.9420001

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Received: 27 March 2024
Revised: 09 April 2024
Accepted: 11 April 2024
Published: 23 May 2024
© National R & D Center for Edible Fungus Processing Technology 2024. Published by Tsinghua University Press.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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