Neuroprotective effects of adipose-derived stem cells on ferrous sulfate-induced neurotoxicity

Qian Wu; Chao Pan; Yang Hu; Gaigai Li; Shiling Chen; Jie Jing; Jingfei Yang; Zhouping Tang

doi:10.26599/BSA.2021.9050008

Brain Science Advances 2021, 7(3): 172-183 https://doi.org/10.26599/BSA.2021.9050008

Research Article |

Open Access | Issue | Published: 08 December 2021

Neuroprotective effects of adipose-derived stem cells on ferrous sulfate-induced neurotoxicity

Show Author's Information Hide Author's Information Qian Wu, Chao Pan, Yang Hu, Gaigai Li, Shiling Chen, Jie Jing, Jingfei Yang, Zhouping Tang(

)

Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

Keywords:

stroke, cytokines, mesenchymal stromal cells, paracrine communication

Cite this article:

Wu Q, Pan C, Hu Y, et al. Neuroprotective effects of adipose-derived stem cells on ferrous sulfate-induced neurotoxicity. Brain Science Advances, 2021, 7(3): 172-183. https://doi.org/10.26599/BSA.2021.9050008

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Abstract Full text About this article

Abstract

Background:

Ferrous ion, a degradation product of hematomas, induces inflammatory reactions and other secondary injuries after intracerebral hemorrhage (ICH). Our study aimed to investigate the specific neuroprotective mechanism of adipose-derived stem cells (ADSCs) on ferrous ion-induced neural injury in vitro.

Methods:

ADSCs were co-cultured with primary cortical neurons in a transwell system treated with ferrous sulfate to generate an in vitro ICH model. ADSCs and cortical neurons were cultured in the upper and lower chambers, respectively. Neuron apoptosis was determined by flow cytometry. The levels of insulin-like growth factor-1 (IGF-1), malondialdehyde (MDA) and nitric oxide synthase (NOS) activity in neuron culture medium were detected with commercial kits. In neurons, protein expression in phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt) signaling pathway, nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway and apoptosis-related proteins were detected by western blot.

Results:

ADSCs attenuated neural apoptosis, reduced MDA levels and NOS activity induced by ferrous sulfate. In neurons, IGF-1 was increased, as were p-PI3K, p-Akt, Nrf2, HO-1, and Bcl-2 while cleaved caspase 3 was down-regulated.

Conclusions:

ADSCs exert neuroprotective effects against ferrous iron-induced neuronal damage by secreting IGF-1 and increasing the levels of Akt-dependent Nrf2/ARE signaling pathway.

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About this article

Neuroprotective effects of adipose-derived stem cells on ferrous sulfate-induced neurotoxicity

Show Author's information Hide Author's Information Qian Wu, Chao Pan, Yang Hu, Gaigai Li, Shiling Chen, Jie Jing, Jingfei Yang, Zhouping Tang(

)

Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

Abstract

Background:

Methods:

Results:

Conclusions:

ADSCs exert neuroprotective effects against ferrous iron-induced neuronal damage by secreting IGF-1 and increasing the levels of Akt-dependent Nrf2/ARE signaling pathway.

Keywords: stroke, cytokines, mesenchymal stromal cells, paracrine communication

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Acknowledgements

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Publication history

Received: 16 January 2021

Revised: 08 March 2021

Accepted: 29 March 2021

Published: 08 December 2021

Issue date: September 2021

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Acknowledgements

We thank Dr. Teng Zhang and Yizheng Huang for technical assistance.

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This article is published with open access at journals.sagepub.com/home/BSA

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