Aging-related alternative splicing landscapes across human T cells

As age-related diseases escalate, deciphering molecular mechanism of immune aging is vital. T cells, crucial in adaptive immunity, undergo aging-related transformations in quantity and quality. The interconnection between aging and alternative splicing of gene expression in different T cell subtype is still unclear. Thus, we examined age-related gene alternative splicing in numerous immune cell subgroups, constructing an aging-associated atlas for alternative splicing across human T cell subtypes. Our study identified numerous age-related alternative splicing events in genes linked to T cell activation, differentiation, migration, and apoptosis. Genes like PDCD4 and ARCN1 with age group-specific alternative splicing events and implicated in T cell aging hint at potential therapeutic targets for immune aging. Overall, our findings present a comprehensive alternative splicing atlas for healthy aging-related molecular programs, introducing fresh perspectives for T cell transformation regulation during aging, and inspiring new approaches for novel T cell aging intervention molecules and methods.