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Review | Open Access

Methods for monitoring cancer cell pyroptosis

Shuo Wang1,*, Yuantong Liu1,*, Lu Zhang1 ( ), Zhijun Sun1,2 ( )
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine, Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China
Department of Oral Maxillofacial-Head Neck Oncology, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China

*These authors contributed equally to this work.

Show Author Information

Abstract

Pyroptosis is a form of proinflammatory cell death that depends on the gasdermin family of proteins. The main features of pyroptosis are altered membrane permeability, cell swelling, membrane rupture, and the ability to mobilize a strong immune response. The relationship between pyroptosis and cancer has become a popular topic in immunological research. Multiple strategies for inducing pyroptosis in cancer cells have been developed for cancer therapy, including chemotherapy, small molecule drugs, and nanomedicines. In this review, we systematically discuss recent advances in research on the mechanisms of pyroptosis, and compare pyroptosis with apoptosis and necroptosis from several aspects. The development of various experimental systems has accompanied rapid progress in this field, but little consensus on monitoring pyroptosis is currently available. We focus on techniques commonly used to monitor pyroptosis, and describe future techniques that may be used to increase our knowledge in this field. Overall, the advancement of pyroptosis detection methods will help researchers to better investigate the relationships between pyroptosis and various cancers, and should provide insights into the use of these promising tools for cancer treatments.

Keywords

cancer immunotherapyPyroptosiscaspasegasdermincell death

References

1

Hodi FS, O’Day SJ, McDermott DF, Weber RW, Sosman JA, Haanen JB, et al. Improved survival with ipilimumab in patients with metastatic melanoma. N Engl J Med. 2010; 363: 711-23.
Crossref Google Scholar
2

Tumeh PC, Harview CL, Yearley JH, Shintaku IP, Taylor EJ, Robert L, et al. PD-1 blockade induces responses by inhibiting adaptive immune resistance. Nature. 2014; 515: 568-71.
Crossref Google Scholar
3

Chen DS, Mellman I. Elements of cancer immunity and the cancerimmune set point. Nature. 2017; 541: 321-30.
Crossref Google Scholar
4

Tang H, Li H, Sun Z. Targeting myeloid-derived suppressor cells for cancer therapy. Cancer Biol Med. 2021; Doi: 10.20892/j.issn.2095-3941.2020.0806.
Crossref Google Scholar
5

Xu Z, Huang X. Cellular immunotherapy for hematological malignancy: recent progress and future perspectives. Cancer Biol Med. 2021; Doi: 10.20892/j.issn.2095-3941.2020.0801.
Crossref Google Scholar
6

Hegde PS, Chen DS. Top 10 challenges in cancer immunotherapy. Immunity. 2020; 52: 17-35.
Crossref Google Scholar
7

Bertheloot D, Latz E, Franklin BS. Necroptosis, pyroptosis and apoptosis: an intricate game of cell death. Cell Mol Immunol. 2021; 18: 1106-21.
Crossref Google Scholar
8

Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation. Cell. 2011; 144: 646.
Crossref Google Scholar
9

Broz P, Pelegrín P, Shao F. The gasdermins, a protein family executing cell death and inflammation. Nat Rev Immunol. 2020; 20: 143-57.
Crossref Google Scholar
10

Friedlander A. Macrophages are sensitive to anthrax lethal toxin through an acid-dependent process. J Biol Chem. 1986; 261: 7123-6.
Crossref Google Scholar
11

Brennan M, Cookson B. Salmonella induces macrophage death by caspase-1-dependent necrosis. Mol Microbiol. 2000; 38: 31-40.
Crossref Google Scholar
12

Cookson BT, Brennan MA. Pro-inflammatory programmed cell death. Trends Microbiol. 2001; 9: 113-4.
Crossref Google Scholar
13

Boise LH, Collins CM. Salmonella-induced cell death: apoptosis, necrosis or programmed cell death? Trends Microbiol. 2001; 9: 64-7.
Crossref Google Scholar
14

Kayagaki N, Warming S, Lamkanfi M, Vande Walle L, Louie S, Dong J, et al. Non-canonical inflammasome activation targets caspase-11. Nature. 2011; 479: 117-21.
Crossref Google Scholar
15

Shi J, Zhao Y, Wang Y, Gao W, Ding J, Li P, et al. Inflammatory caspases are innate immune receptors for intracellular LPS. Nature. 2014; 514: 187-92.
Crossref Google Scholar
16

Shi J, Zhao Y, Wang K, Shi X, Wang Y, Huang H, et al. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death. Nature. 2015; 526: 660-5.
Crossref Google Scholar
17

Kayagaki N, Stowe I, Lee B, O’Rourke K, Anderson K, Warming S, et al. Caspase-11 cleaves gasdermin D for non-canonical inflammasome signalling. Nature. 2015; 526: 666-71.
Crossref Google Scholar
18

He W, Wan H, Hu L, Chen P, Wang X, Huang Z, et al. Gasdermin D is an executor of pyroptosis and required for interleukin-1β secretion. Cell Res. 2015; 25: 1285-98.
Crossref Google Scholar
19

Liu X, Zhang Z, Ruan J, Pan Y, Magupalli V, Wu H, et al. Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores. Nature. 2016; 535: 153-8.
Crossref Google Scholar
20

Ding J, Wang K, Liu W, She Y, Sun Q, Shi J, et al. Pore-forming activity and structural autoinhibition of the gasdermin family. Nature. 2016; 535: 111-6.
Crossref Google Scholar
21

Chen X, He W, Hu L, Li J, Fang Y, Wang X, et al. Pyroptosis is driven by non-selective gasdermin-D pore and its morphology is different from MLKL channel-mediated necroptosis. Cell Res. 2016; 26: 1007-20.
Crossref Google Scholar
22

Aglietti R, Estevez A, Gupta A, Ramirez M, Liu P, Kayagaki N, et al. GsdmD p30 elicited by caspase-11 during pyroptosis forms pores in membranes. Proc Natl Acad Sci U S A. 2016; 113: 7858-63.
Crossref Google Scholar
23

Wang Y, Gao W, Shi X, Ding J, Liu W, He H, et al. Chemotherapy drugs induce pyroptosis through caspase-3 cleavage of a gasdermin. Nature. 2017; 547: 99-103.
Crossref Google Scholar
24

Wu D, Wang S, Yu G, Chen X. Cell death mediated by the pyroptosis pathway with the aid of nanotechnology: prospects for cancer therapy. Angew Chem Int Ed. 2020; 60: 8018-34.
Crossref Google Scholar
25

Yu P, Zhang X, Liu N, Tang L, Peng C, Chen X. Pyroptosis: mechanisms and diseases. Signal Transduct Target Ther. 2021; 6: 128.
Crossref Google Scholar
26

Wang M, Jiang S, Zhang Y, Li P, Wang K. The multifaceted roles of pyroptotic cell death pathways in cancer. Cancers. 2019; 11: 1313.
Crossref Google Scholar
27

Wang Q, Wang Y, Ding J, Wang C, Zhou X, Gao W, et al. A bioorthogonal system reveals antitumour immune function of pyroptosis. Nature. 2020; 579: 421-6.
Crossref Google Scholar
28

Lamkanfi M, Dixit VM. Mechanisms and functions of inflammasomes. Cell. 2014; 157: 1013-22.
Crossref Google Scholar
29

Lugrin J, Martinon F. The AIM2 inflammasome: sensor of pathogens and cellular perturbations. Immunol Rev. 2018; 281: 99-114.
Crossref Google Scholar
30

Miao EA, Leaf IA, Treuting PM, Mao DP, Dors M, Sarkar A, et al. Caspase-1-induced pyroptosis is an innate immune effector mechanism against intracellular bacteria. Nat Immunol. 2010; 11: 1136-42.
Crossref Google Scholar
31

Broz P, Dixit V. Inflammasomes: mechanism of assembly, regulation and signalling. Nat Rev Immunol. 2016; 16: 407-20.
Crossref Google Scholar
32

Vanaja S, Rathinam V, Fitzgerald K. Mechanisms of inflammasome activation: recent advances and novel insights. Trends Cell Biol. 2015; 25: 308-15.
Crossref Google Scholar
33

Boucher D, Monteleone M, Coll R, Chen K, Ross C, Teo J, et al. Caspase-1 self-cleavage is an intrinsic mechanism to terminate inflammasome activity. J Exp Med. 2018; 215: 827-40.
Crossref Google Scholar
34

Miao EA, Mao DP, Yudkovsky N, Bonneau R, Lorang CG, Warren SE, et al. Innate immune detection of the type Ⅲ secretion apparatus through the NLRC4 inflammasome. Proc Natl Acad Sci U S A. 2010; 107: 3076-80.
Crossref Google Scholar
35

Sborgi L, Rühl S, Mulvihill E, Pipercevic J, Heilig R, Stahlberg H, et al. GSDMD membrane pore formation constitutes the mechanism of pyroptotic cell death. EMBO J. 2016; 35: 1766-78.
Crossref Google Scholar
36

Yang J, Liu Z, Wang C, Yang R, Rathkey J, Pinkard O, et al. Mechanism of gasdermin D recognition by inflammatory caspases and their inhibition by a gasdermin D-derived peptide inhibitor. Proc Natl Acad Sci U S A. 2018; 115: 6792-7.
Crossref Google Scholar
37

Xia S, Zhang Z, Magupalli V, Pablo J, Dong Y, Vora S, et al. Gasdermin D pore structure reveals preferential release of mature interleukin-1. Nature. 2021; 593: 607-11.
Crossref Google Scholar
38

Baker PJ, Boucher D, Bierschenk D, Tebartz C, Whitney PG, D’Silva DB, et al. NLRP3 inflammasome activation downstream of cytoplasmic LPS recognition by both caspase-4 and caspase-5. Eur J Immunol. 2015; 45: 2918-26.
Crossref Google Scholar
39

Rühl S, Broz P. Caspase-11 activates a canonical NLRP3 inflammasome by promoting K(+) efflux. Eur J Immunol. 2015; 45: 2927-36.
Crossref Google Scholar
40

Davis M, Fairgrieve M, Den Hartigh A, Yakovenko O, Duvvuri B, Lood C, et al. Calpain drives pyroptotic vimentin cleavage, intermediate filament loss, and cell rupture that mediates immunostimulation. Proc Natl Acad Sci U S A. 2019; 116: 5061-70.
Crossref Google Scholar
41

Rühl S, Shkarina K, Demarco B, Heilig R, Santos J, Broz P. ESCRTdependent membrane repair negatively regulates pyroptosis downstream of GSDMD activation. Science. 2018; 362: 956-60.
Crossref Google Scholar
42

Yang D, He Y, Muñoz-Planillo R, Liu Q, Núñez G. Caspase-11 requires the pannexin-1 channel and the purinergic P2X7 pore to mediate pyroptosis and endotoxic shock. Immunity. 2015; 43: 923-32.
Crossref Google Scholar
43

Chen K, Demarco B, Broz P. Pannexin-1 promotes NLRP3 activation during apoptosis but is dispensable for canonical or noncanonical inflammasome activation. Eur J Immunol. 2020; 50: 170-7.
Crossref Google Scholar
44

Chen K, Demarco B, Heilig R, Shkarina K, Boettcher A, Farady C, et al. Extrinsic and intrinsic apoptosis activate pannexin-1 to drive NLRP3 inflammasome assembly. EMBO J. 2019; 38: e101638.
Crossref Google Scholar
45

Evavold C, Hafner-Bratkovič I, Devant P, D’Andrea J, Ngwa E, Boršić E, et al. Control of gasdermin D oligomerization and pyroptosis by the Ragulator-Rag-mTORC1 pathway. Cell. 2021; 184: 4495-511.
Crossref Google Scholar
46

Rogers C, Fernandes-Alnemri T, Mayes L, Alnemri D, Cingolani G, Alnemri E. Cleavage of DFNA5 by caspase-3 during apoptosis mediates progression to secondary necrotic/pyroptotic cell death. Nat Commun. 2017; 8: 14128.
Crossref Google Scholar
47

Orning P, Weng D, Starheim K, Ratner D, Best Z, Lee B, et al. Pathogen blockade of TAK1 triggers caspase-8-dependent cleavage of gasdermin D and cell death. Science. 2018; 362: 1064-9.
Crossref Google Scholar
48

Sarhan J, Liu BC, Muendlein HI, Li P, Nilson R, Tang AY, et al. Caspase-8 induces cleavage of gasdermin D to elicit pyroptosis during Yersinia infection. Proc Natl Acad Sci U S A. 2018; 115: E10888-97.
Crossref Google Scholar
49

Zheng Z, Deng W, Bai Y, Miao R, Liu X. The lysosomal RagRagulator complex licenses RIPK1– and caspase-8–mediated pyroptosis by yersinia. Science. 2021; 372: eabg0269.
Crossref Google Scholar
50

Hou J, Zhao R, Xia W, Chang CW, You Y, Hsu JM, et al. PD-L1-mediated gasdermin C expression switches apoptosis to pyroptosis in cancer cells and facilitates tumour necrosis. Nat Cell Biol. 2020; 22: 1264-75.
Crossref Google Scholar
51

Zhou Z, He H, Wang K, Shi X, Wang Y, Su Y, et al. Granzyme a from cytotoxic lymphocytes cleaves GSDMB to trigger pyroptosis in target cells. Science. 2020; 368: eaaz7548.
Crossref Google Scholar
52

Liu Y, Fang Y, Chen X, Wang Z, Liang X, Zhang T, et al. Gasdermin E-mediated target cell pyroptosis by CAR T cells triggers cytokine release syndrome. Sci Immunol. 2020; 5: eaax7969.
Crossref Google Scholar
53

Zhang Z, Zhang Y, Xia S, Kong Q, Li S, Liu X, et al. Gasdermin e suppresses tumour growth by activating anti-tumour immunity. Nature. 2020; 579: 415-20.
Crossref Google Scholar
54

Snyder AG, Oberst A. The antisocial network: cross talk between cell death programs in host defense. Annu Rev Immunol. 2021; 39: 77-101.
Crossref Google Scholar
55

Fink S, Cookson B. Apoptosis, pyroptosis, and necrosis: mechanistic description of dead and dying eukaryotic cells. Infect Immun. 2005; 73: 1907-16.
Crossref Google Scholar
56

Galluzzi L, Vitale I, Aaronson SA, Abrams JM, Adam D, Agostinis P, et al. Molecular mechanisms of cell death: recommendations of the nomenclature committee on cell death 2018. Cell Death Differ. 2018; 25: 486-541.
Crossref Google Scholar
57

Kesavardhana S, Malireddi RKS, Kanneganti TD. Caspases in cell death, inflammation, and pyroptosis. Annu Rev Immunol. 2020; 38: 567-95.
Crossref Google Scholar
58

Jorgensen I, Miao EA. Pyroptotic cell death defends against intracellular pathogens. Immunol Rev. 2015; 265: 130-42.
Crossref Google Scholar
59

Bergsbaken T, Cookson BT. Macrophage activation redirects yersinia-infected host cell death from apoptosis to caspase-1-dependent pyroptosis. PLoS Pathog. 2007; 3: e161.
Crossref Google Scholar
60

Tsuchiya K. Switching from apoptosis to pyroptosis: gasderminelicited inflammation and antitumor immunity. Int J Mol Sci. 2021; 22: 426.
Crossref Google Scholar
61

Frank D, Vince J. Pyroptosis versus necroptosis: similarities, differences, and crosstalk. Cell Death Differ. 2019; 26: 99-114.
Crossref Google Scholar
62

Kaiser WJ, Upton JW, Long AB, Livingston-Rosanoff D, DaleyBauer LP, Hakem R, et al. RIP3 mediates the embryonic lethality of caspase-8-deficient mice. Nature. 2011; 471: 368-72.
Crossref Google Scholar
63

Oberst A, Dillon CP, Weinlich R, McCormick LL, Fitzgerald P, Pop C, et al. Catalytic activity of the caspase-8-FLIP(L) complex inhibits RIPK3-dependent necrosis. Nature. 2011; 471: 363-7.
Crossref Google Scholar
64

Li Z, Huang Z, Zhang H, Lu J, Tian Y, Wei Y, et al. P2X7 receptor induces pyroptotic inflammation and cartilage degradation in osteoarthritis via NF-κB/NLRP3 crosstalk. Oxid Med Cell Longev. 2021; 2021: 8868361.
Crossref Google Scholar
65

Xie W, Ding J, Xie X, Yang X, Wu X, Chen Z, et al. Hepatitis B virus X protein promotes liver cell pyroptosis under oxidative stress through NLRP3 inflammasome activation. Inflamm Res. 2020; 69: 683-96.
Crossref Google Scholar
66

Yang W, Liu S, Li Y, Wang Y, Deng Y, Sun W, et al. Pyridoxine induces monocyte-macrophages death as specific treatment of acute myeloid leukemia. Cancer Lett. 2020; 492: 96-105.
Crossref Google Scholar
67

Hu JJ, Liu X, Xia S, Zhang Z, Zhang Y, Zhao J, et al. FDA-approved disulfiram inhibits pyroptosis by blocking gasdermin D pore formation. Nat Immunol. 2020; 21: 736-45.
Crossref Google Scholar
68

Xia S, Ruan J, Wu H. Monitoring gasdermin pore formation in vitro. Methods Enzymol. 2019; 625: 95-107.
Crossref Google Scholar
69

Mulvihill E, Sborgi L, Mari S, Pfreundschuh M, Hiller S, Müller D. Mechanism of membrane pore formation by human gasdermin-D. EMBO J. 2018; 37: e98321.
Crossref Google Scholar
70

Liu Y, Zhang T, Zhou Y, Li J, Liang X, Zhou N, et al. Visualization of perforin/gasdermin/complement-formed pores in real cell membranes using atomic force microscopy. Cell Mol Immunol. 2019; 16: 611-20.
Crossref Google Scholar
71

Liu L, Sha R, Yang L, Zhao X, Zhu Y, Gao J, et al. Impact of morphology on iron oxide nanoparticles-induced inflammasome activation in macrophages. ACS Appl Mater Inter. 2018; 10: 41197-206.
Crossref Google Scholar
72

Du Q, Ge D, Mirshafiee V, Chen C, Li M, Xue C, et al. Assessment of neurotoxicity induced by different-sized stober silica nanoparticles: induction of pyroptosis in microglia. Nanoscale. 2019; 11: 12965-72.
Crossref Google Scholar
73

Duan WR, Garner DS, Williams SD, Funckes-Shippy CL, Spath IS, Blomme EA. Comparison of immunohistochemistry for activated caspase-3 and cleaved cytokeratin 18 with the TUNEL method for quantification of apoptosis in histological sections of PC-3 subcutaneous xenografts. J Pathol. 2003; 199: 221-8.
Crossref Google Scholar
74

Jiang L, Poon I. Methods for monitoring the progression of cell death, cell disassembly and cell clearance. Apoptosis. 2019; 24: 208-20.
Crossref Google Scholar
75

Volchuk A, Ye A, Chi L, Steinberg BE, Goldenberg NM. Indirect regulation of HMGB1 release by gasdermin D. Nat Commun. 2020; 11: 4561.
Crossref Google Scholar
76

Russo AJ, Vasudevan SO, Mendez-Huergo SP, Kumari P, Menoret A, Duduskar S, et al. Intracellular immune sensing promotes inflammation via gasdermin D-driven release of a lectin alarmin. Nat Immunol. 2021; 22: 154-65.
Crossref Google Scholar
77

Mirshafiee V, Sun B, Chang C, Liao Y, Jiang W, Jiang J, et al. Toxicological profiling of metal oxide nanoparticles in liver context reveals pyroptosis in Kupffer cells and macrophages versus apoptosis in hepatocytes. ACS Nano. 2018; 12: 3836-52.
Crossref Google Scholar
78

Garrod KR, Moreau HD, Garcia Z, Lemaitre F, Bouvier I, Albert ML, et al. Dissecting T cell contraction in vivo using a genetically encoded reporter of apoptosis. Cell Rep. 2012; 2: 1438-47.
Crossref Google Scholar
79

Posson DJ, Rusinova R, Andersen OS, Nimigean CM. Stopped-flow fluorometric ion flux assay for ligand-gated ion channel studies. Methods Mol Biol. 2018; 1684: 223-35.
Crossref Google Scholar
80

Zhang C, Li C, Wang Y, Xu L, He X, Zeng Q, et al. Chemotherapeutic paclitaxel and cisplatin differentially induce pyroptosis in A549 lung cancer cells via caspase-3/GSDME activation. Apoptosis. 2019; 24: 312-25.
Crossref Google Scholar
81

Wang Y, Yin B, Li D, Wang G, Han X, Sun X. GSDME mediates caspase-3-dependent pyroptosis in gastric cancer. Biochem Biophys Res Commun. 2018; 495: 1418-25.
Crossref Google Scholar
82

Galluzzi L, Kroemer G. Secondary necrosis: accidental no more. Trends Cancer. 2017; 3: 1-2.
Crossref Google Scholar
83

Fink SL, Cookson BT. Caspase-1-dependent pore formation during pyroptosis leads to osmotic lysis of infected host macrophages. Cell Microbiol. 2006; 8: 1812-25.
Crossref Google Scholar
84

Ploetz E, Zimpel A, Cauda V, Bauer D, Lamb D, Haisch C, et al. Metal-organic framework nanoparticles induce pyroptosis in cells controlled by the extracellular PH. Adv Mater. 2020; 32: e1907267.
Crossref Google Scholar
85

Bedoui S, Herold M, Strasser A. Emerging connectivity of programmed cell death pathways and its physiological implications. Nat Rev Mol Cell Biol. 2020; 21: 678-95.
Crossref Google Scholar
86

Lu Y, Xu S, Chen H, He M, Deng Y, Cao Z, et al. CdSe/ZnS quantum dots induce hepatocyte pyroptosis and liver inflammation via NLRP3 inflammasome activation. Biomaterials. 2016; 90: 27-39.
Crossref Google Scholar
87

Wang D, Zheng J, Hu Q, Zhao C, Chen Q, Shi P, et al. Magnesium protects against sepsis by blocking gasdermin D N-terminalinduced pyroptosis. Cell Death Differ. 2020; 27: 466-81.
Crossref Google Scholar
88

Zhao P, Wang M, Chen M, Chen Z, Peng X, Zhou F, et al. Programming cell pyroptosis with biomimetic nanoparticles for solid tumor immunotherapy. Biomaterials. 2020; 254: 120142.
Crossref Google Scholar
89

Wang K, Sun Q, Zhong X, Zeng M, Zeng H, Shi X, et al. Structural mechanism for GSDMD targeting by autoprocessed caspases in pyroptosis. Cell. 2020; 180: 941-55 e20.
Crossref Google Scholar
90

Vanden Berghe T, Grootjans S, Goossens V, Dondelinger Y, Krysko DV, Takahashi N, et al. Determination of apoptotic and necrotic cell death in vitro and in vivo. Methods. 2013; 61: 117-29.
Crossref Google Scholar
91

Muendlein H, Jetton D, Connolly W, Eidell K, Magri Z, Smirnova I, et al. cFLIPL protects macrophages from LPS-induced pyroptosis via inhibition of complex Ⅱ formation. Science. 2020; 367: 1379-84.
Crossref Google Scholar
92

Ruan J, Wang S, Wang J. Mechanism and regulation of pyroptosismediated in cancer cell death. Chem Biol Interact. 2020; 323: 109052.
Crossref Google Scholar
93

Galon J, Bruni D. Approaches to treat immune hot, altered and cold tumours with combination immunotherapies. Nat Rev Drug Discov. 2019; 18: 197-218.
Crossref Google Scholar
94

Liu Y, Sun Z. Turning cold tumors into hot tumors by improving T-cell infiltration. Theranostics. 2021; 11: 5365-86.
Crossref Google Scholar
95

Karki R, Sharma B, Tuladhar S, Williams E, Zalduondo L, Samir P, et al. Synergism of TNF-α and IFN-γ triggers inflammatory cell death, tissue damage, and mortality in SARS-CoV-2 infection and cytokine shock syndromes. Cell. 2021; 184: 149-68.e17.
Crossref Google Scholar
96

Moossavi M, Parsamanesh N, Bahrami A, Atkin SL, Sahebkar A. Role of the NLRP3 inflammasome in cancer. Mol Cancer. 2018; 17: 158.
Crossref Google Scholar
97

Humphries F, Shmuel-Galia L, Ketelut-Carneiro N, Li S, Wang B, Nemmara VV, et al. Succination inactivates gasdermin D and blocks pyroptosis. Science. 2020; 369: 1633-7.
Crossref Google Scholar
98

Alvero AB, Mor GG. Detection of cell death mechanisms: methods and protocols. Methods Mol Biol. 2021; 2255: 149-57.
Crossref Google Scholar
99

Mesa K, Rompolas P, Zito G, Myung P, Sun T, Brown S, et al. Nicheinduced cell death and epithelial phagocytosis regulate hair follicle stem cell pool. Nature. 2015; 522: 94-7.
Crossref Google Scholar
100

Mayer C, Gazumyan A, Kara E, Gitlin A, Golijanin J, Viant C, et al. The microanatomic segregation of selection by apoptosis in the germinal center. Science. 2017; 358: eaao2602.
Crossref Google Scholar
101

Li Q, Shi N, Cai C, Zhang M, He J, Tan Y, et al. The role of mitochondria in pyroptosis. Front Cell Dev Biol. 2020; 8: 630771.
Crossref Google Scholar
Cancer Biology & Medicine
Volume 19 Issue 4,
April 2022
Pages 398-414
DOI: 10.20892/j.issn.2095-3941.2021.0504
Cite this article:
Wang S, Liu Y, Zhang L, et al. Methods for monitoring cancer cell pyroptosis. Cancer Biology & Medicine, 2022, 19(4): 398-414. https://doi.org/10.20892/j.issn.2095-3941.2021.0504

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Received: 08 September 2021
Accepted: 01 November 2021
Published: 01 April 2022
©2022 Cancer Biology & Medicine.

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