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Basic Research | Open Access

Laser photocoagulation improves the transduction efficiency of lentivirus vectors injected intravitreally

Jian Jiao1( ), Yi-Jun Xu1, Xue-Dong Li2, Wen Hua2, Yan-Rong Jiang3
Department of Ophthalmology, People’s Hospital of Beijing Daxing District, Beijing 102600, China
Department of Ophthalmology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China
Department of Ophthalmology, People’s Hospital, Peking University People’s Hospital, Beijing 100044, China
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Abstract

AIM

To evaluate the effect of laser photocoagulation on transfection efficiency of lentivirus (LV) vectors injected intravitreally.

METHODS

The rat retina was photocoagulated prior to intravitreal injection of LV vectors encoding red fluorescent protein. Rats given intravitreal injection or photocoagulation alone served as controls. The transduction efficiency was analyzed by fundus angiography, histopathology, and immunofluorescence staining. Potential adverse effect of laser photocoagulation was evaluated in choroid flat mounts by observing the morphology of retinal pigment epithelium (RPE) that formed the outer blood-retina barrier.

RESULTS

Laser photocoagulation enhanced the retinal area transduced by LV vectors in fundus images and increased the maximum transduction thickness in histopathological sections. Choroid flat mounts showed the RPE transduced by LV vectors maintained normal morphology.

CONCLUSION

Laser photocoagulation improves the transduction efficiency of LV vectors injected intravitreally, identifying this as a promising gene therapy strategy for inherited retinal dystrophies caused by gene mutations in RPE and photoreceptor cells.

Keywords

gene therapylaser photocoagulationlentivirus vectortransduction efficiencyintravitreal injection

References

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International Journal of Ophthalmology
Volume 19 Issue 6,
June 2026
Pages 1057-1064
DOI: 10.18240/ijo.2026.06.05

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Cite this article:
Jiao J, Xu Y-J, Li X-D, et al. Laser photocoagulation improves the transduction efficiency of lentivirus vectors injected intravitreally. International Journal of Ophthalmology, 2026, 19(6): 1057-1064. https://doi.org/10.18240/ijo.2026.06.05

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Received: 15 March 2025
Accepted: 25 February 2026
Published: 18 June 2026
© 2026 International Journal of Ophthalmology Press

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).