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Basic Research | Open Access

Hepatocyte growth factor promotes retinal pigment epithelium cell activity through MET/AKT signaling pathway

Si-Rui Zhou1,2,3,4, Yu-Sheng Zhu1,2,3,4, Wen-Ting Yuan1,2,3,4, Xiao-Yan Pan1,2,3,4, Tong Wang1,2,3,4( ), Xiao-Dong Chen1,2,3,4( )
Faculty of Life Sciences and Medicine, Northwest University, Xi’an 710069, Shaanxi Province, China
First Affiliated Hospital of Northwest University, Northwest University, Xi’an 710069, Shaanxi Province, China
Department of Ophthalmology, Xi’an No.1 Hospital, Xi’an 710002, Shaanxi Province, China
Shaanxi Institute of Ophthalmology, Shaanxi Provincial Key Lab of Ophthalmology, Clinical Research Center for Ophthalmology Diseases of Shaanxi Province, Xi’an 710002, Shaanxi Province, China
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Abstract

AIM

To explore the effects of hepatocyte growth factor (HGF) on retinal pigment epithelium (RPE) cell behaviors.

METHODS

The human adult retinal pigment epithelial cell line-19 (ARPE-19) were treated by HGF or mesenchymal-epithelial transition factor (MET) inhibitor SU11274 in vitro. Cell viability was detected by a Cell Counting Kit-8 assay. Cell proliferation and motility was detected by a bromodeoxyuridine incorporation assay and a wound healing assay, respectively. The expression levels of MET, phosphorylated MET, protein kinase B (AKT), and phosphorylated AKT proteins were determined by Western blot assay. The MET and phosphorylated MET proteins were also determined by immunofluorescence assay.

RESULTS

HGF increased ARPE-19 cells’ viability, proliferation and migration, and induced an increase of phosphorylated MET and phosphorylated AKT proteins. SU11274 significantly reduced cell viability, proliferation, and migration and decreased the expression of MET and AKT proteins. SU11274 suppressed HGF-induced increase of viability, proliferation, and migration in ARPE-19 cells. Additionally, SU11274 also blocked HGF-induced phosphorylation of MET and AKT proteins.

CONCLUSION

HGF enhances cellular viability, proliferation, and migration in RPE cells through the MET/AKT signaling pathway, whereas this enhancement is suppressed by the MET inhibitor SU11274. HGF-induced MET/AKT signaling might be a vital contributor of RPE cells survival.

Keywords

hepatocyte growth factormesenchymal epithelial transition factorSU11274retinal pigment epithelial cells

References

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International Journal of Ophthalmology
Volume 17 Issue 5,
May 2024
Pages 806-814
DOI: 10.18240/ijo.2024.05.03

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Cite this article:
Zhou S-R, Zhu Y-S, Yuan W-T, et al. Hepatocyte growth factor promotes retinal pigment epithelium cell activity through MET/AKT signaling pathway. International Journal of Ophthalmology, 2024, 17(5): 806-814. https://doi.org/10.18240/ijo.2024.05.03

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Received: 31 October 2023
Accepted: 30 January 2024
Published: 18 May 2024
© 2024 International Journal of Ophthalmology Press

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).