Determination of Scopolamine, Anisodamine and Atropine in Blood and Urine by SLE-HPLC-MS/MS

This paper aims to establish a solid-phase support liquid-liquid extraction-high performance liquid chromatography/triple quadrupole tandem mass spectrometry (SLE-HPLC-MS/MS) method for the detection of scopolamine, anisodamine and atropine in blood and urine. The effects of protein precipitated method, solid phase extraction method and solid-phase supported liquid-liquid extraction method on the extraction of drugs from blood and urine samples were investigated. Scopolamine, anisodamine and atropine, in blood and urine samples were analyzed by high performance liquid chromatography triple quadrupole tandem mass spectrometry. The results showed that the recovery rate of solid-phase supported liquid-liquid extraction was the highest. The linear relationship between the concentration of scopolamine, anisodamine and atropine in blood and urine and the peak area (r>0.9992) was good in the range of 0.1－100 ng/mL (blood) and 0.5－100 ng/mL (urine). The minimum detection limit of scopolamine, anisodamine and atropine in blood was 0.01 ng/mL, and the quantitative limit was 0.1ng/mL; The minimum detection limit of scopolamine, anisodamine and atropine in urine was 0.05 ng/mL, and the quantification limit was 0.5 ng/mL. The solid-phase supported liquid-liquid extraction liquid chromatography/tandem mass spectrometry method is characterized by simple operation, less solvent usage and high recovery rate. It is suitable in the detection of scopolamine, anisodamine and atropine in blood and urine for three types of drug poisoning cases (incidents).