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As one of the most commonly used reagents for amino acid detection, ninhydrin has a wide range of applications in forensic science and is a classic and effective method for displaying old fingerprints on permeable objects. However, the traditional ninhydrin display method still needs further improvement in the display effect of latent fingerprints on objects with complex background colors. Based on a review of relevant research results at home and abroad, a brief review was conducted on the composition of fingerprint substances, the mechanism of ninhydrin in fingerprint development, and the improvement of traditional ninhydrin development methods. A detailed review was also provided on the methods for enhancing the development effect since ninhydrin was applied in the field of fingerprint development. The innovation of the traditional ninhydrin solution method for enhancing visualization mainly manifests in three aspects: 1) optimization of ninhydrin solution method reagent formula, such as screening of the best solvent, exploring the optimal concentration, and discussing the influence of pH value on visualization effect; 2) The innovation of ninhydrin display methods, such as solid medium method, ninhydrin vacuum fumigation method, spray display method, etc., mainly solves the problems of carbonization interference and background ink interference in thermosensitive paper; 3) The fingerprint enhancement treatment using ninhydrin, mainly includes metal salt enhancement, rare earth-Ruhmann's Purple violet coordination compound enhancement, and trypsin enhancement. The metal salt enhancement method and the rare earth-Ruhmann's Purple coordination compound enhancement method have great research potential in the future development trend of latent fingerprints.
This is an open access article under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0, http://creativecommons.org/licenses/by/4.0/).
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