Study on the Secondary Metabolites of Marine-Derived Fungus Trichoderma virens

This study focused on secondary metabolites from fungus of Trichoderma virens. The fungus was isolated from marine sediment in the Northwest Pacific Ocean, with a sampling depth of over 5 km. Multiple modern separation methods were adopted in this paper, and chemical components and pro-angiogenic activities of fungus were investigated systematically.Structural information of all compounds was determined by multiple spectroscopy technologies, including UV, NMR and MS. Further structural verification was completed through the comparison of published literature. A total of ten compounds were identified, including eight terpenoids and two alkaloids.All isolated compounds are listed as follows: rhinomilisin B (1), heptelidic acid chlorohydrin (2), (5aS, 6R, 9S, 9aS)-9-hydroxy-6-isopropyl-9-(hydroxymethyl)-1, 3, 5a, 6, 7, 8, 9, 9a-octahydro-1-oxobenzoxepine-4-carboxylic acid (3), trichocadinin A (4), harzianone A (5), CAF-603 (6), trichocarotin M (7), (3S, 6R)-6-(para-hydroxybenzyl)-1, 4-dimethyl-3, 6-bis(methylthio)piperazine-2, 5-dione (8), didehydrobisdethiobis(methylthio)gliotoxin (9), ethyl hydroheptelidate (10). The pro-angiogenic activity was tested through a zebrafish model. At 25 μmol/L, compounds 1—7 and 9 obviously accelerated the growth of intersegmental vessels in zebrafish. This shows Trichoderma virens can synthesize a variety of secondary metabolites with pro-angiogenic activity.