Research progress on targeted screening of bioactive compounds from traditional Chinese medicine by immobilized target proteins

Drug discovery has always faced the problems such as long cycles and low success rates. The screening and evaluation of the potential compounds from traditional Chinese medicine have become the core breakthrough point and important technical support to solve the above problems. The authors' group innovated the principles of affinity chromatography. They immobilized several target proteins onto solid supports, prepared a series of highly active functional chromatographic stationary phases, and proposed the new concept of targeted screening of bioactive compounds from traditional Chinese medicine by immobilized target proteins. The paper reviews the work from the authors' group during the last five years, which included the methods for the oriented protein immobilization, the targeted screening approaches for bioactive compounds from traditional Chinese medicine, and the chromatographic methods for the early evaluation of potential compounds.Finally, the potential application of the immobilized target proteins in the targeted screening of bioactive compounds from traditional Chinese medicine were also summarized.