Embryo proliferation and maturation culture of Quercus variabilis Blume var. pyramidalis

In order to explore the influence of various factors on the development and maturation of Quercus variabilis Blume var. pyramidalis embryo, and further improve the embryogenesis system of Q. variabilis Blume var. pyramidalis.

The main factors affecting the proliferation and maturation of Q. variabilis Blume var. pyramidalis embryo were explored by using the embryonic callus induced by Q. variabilis Blume var. pyramidalis embryo as materials: single-factor experiments were used to screen the types of light conditions (strong light, weak light, no light) and hormones (naphthaleneacetic acid (NAA), cytokinin 6-benzylaminopurine (6-BA), auxin 2, 4-dichlorophenoxyacetic acid (2, 4-D)) suitable for the proliferation of Q. variabilis Blume var. pyramidalis embryo. The concentration and ratio of hormones affecting embryonic proliferation were analyzed by two-factor tests. Univariate experimental analysis was carried out on sucrose concentration, activated carbon concentration and ABA concentration affecting embryo maturation. Finally, a two-factor test was carried out on the concentration and ratio of hormones affecting embryonic proliferation.

1) Among three different light intensities, the induction rate of embryonic proliferation was the highest in the strong light environment; 2) There were significant differences in the proliferation of embryos by different plant hormone ratios, with the highest proliferation rate of 83.33% with 0.10 mg/L2, 4-D and 0.80 mg/L 6-BA; 3) When the sucrose concentration was 5.00%, the maturity rate of the embryo was the highest which was 77.80%; When the ABA concentration was 0.50 mg/L, the maturity rate reached up to 50.00%; When the concentration of activated carbon was 1.00g/L, the growth was good, and the maturity rate reached 57.10%; 4) When the concentration of NAA and 6-BA was 0.25 mg/L at the same time, the maturity rate of embryo reached up to 90.50%.

1) The body embryo of Q. variabilis Blume var. pyramidalis has a good proliferation effect in a strong light environment; 2) The optimal proliferation medium is: MS + (6.0 g/L) agar + (3.00%) sucrose + 2, 4-D (0.10 mg/L) + 6-BA (0.80 mg/L); 3) The optimal maturation medium is: MS+(7.0 g/L) agar + (5.00%) sucrose + NAA (0.25 mg/L) + 6-BA (0.50 mg/L) + ABA (0.50 mg/L) + activated carbon (1.50 g/L).