Study on Beneficial Characteristics in Vitro of Enterococcus Faecalis EF-ZA1107-06

Studies have shown that probiotic has good antidiabetic and antioxidant activities and great inhibitory effects on human cancer cell lines. This paper took α-glucosidase and α-amylase inhibition rate, superoxide anion radical scavenging rate, ferrous ion chelating rate and reducing activity as indexes, and compared the antidiabetic and antioxidant capacities in vitro between Enterococcus faecalis EF-ZA1107-06 and Lactobacillus rhamnosus GG (LGG). The effects of EF-ZA1107-06 on cellular oxidative stress were investigated by measuring the enzyme activities of glutathione peroxidase (GSH-Px), total superoxide dismutase (T-SOD) and catalase (CAT) in Caco-2 cell lines. The anticancer activity and mechanism were also preliminarily explored using HepG2 and MDA-MB-231 cell lines. The results show that the supernatant of EF-ZA1107-06 has the highest scavenging rate of superoxide anion free radical from 40.78% to 59.61% and strongest ferrous ion chelating ability from 39.28% to 56.59%, while the lysate of EF-ZA1107-06 has the highest reducing activity, equivalent to 1.072 mmol/L equivalent cysteine, which is remarkably higher than that of LGG lysate (P < 0.05). The inhibition of α-amylase and α-glucosidase activity confirms the antidiabetic activity of EF-ZA1107-06. Meanwhile, EF-ZA1107-06 can reduce oxidative damage caused by H₂O₂ to Caco-2 cells to a certain extent and inhibit the S phase and G2 phase of HepG2 cells, but only the S phase of MDA-MB-231 cells. The induction of the early apoptosis of HepG2 cells and MDA-MB-231 cells confirms the anticancer activity of EF-ZA1107-06.