Protective Effect of Schisandra Chinensis Oil on H₂O₂-induced Oxidative Stress Injury in HaCaT Cells by 3D Skin Model

The protective effect of Schisandra chinensis oil on H₂O₂-induced oxidative damage of HaCaT cells is evaluated by 3D skin model. Schisandra chinensis oil was extracted by steam distillation, and its chemical constituents analyzed by GC-MS. And the antioxidant activity of schisandra chinensis oil was evaluated by DPPH and ABTS free radical scavenging experiments, combined with 2D and 3D skin models induced by H₂O₂. The results showed that Schisandra chinensis oil mainly contained 37 compounds including ylenene, β-cimarene and L-α-pinene. Schisandra chinensis oil had good scavenging effect on DPPH and ABTS free radicals, with IC₅₀ of 5.6 mg/mL and 9.4 mg/mL, respectively. What’s more, Schisandra chinensis oil can improve the survival rate of HaCaT cells after H₂O₂ treatment, increasing the epidermal thickness of 3D skin model, decreasing the level of Interleukin-6(IL-6), and up-regulating the activities of SOD and CAT enzymes. The results confirmed that Schisandra chinensis oil had protective effect on H₂O₂-induced oxidative stress injury model of HaCaT cells, which could lay a foundation for the development of anti-aging products of schisandra chinensis oil.