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Basic Study | Publishing Language: Chinese | Open Access

Effects of a red light-emitting diode on the proliferation and osteogenic differentiation of stem cells from the apical papilla

Yutong SU, Lan HOU, Bing JIANG, Genzi ZHENG, Yuan LIU, Yao WANG( )
The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, China
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Abstract

Objective

To explore the effects of red LEDs on the proliferation and osteogenic differentiation of human stem cells from apical papilla (hSCAPs).

Methods

hSCAPs were obtained by isolation, culture and flow cytometry in vitro and irradiated with 1, 3, 5, and 7 J/cm2 red LEDs. The proliferation of hSCAPs was detected using a CCK-8 assay. The osteogenic differentiation of hSCAPs was evaluated using alkaline phosphatase (ALP) staining, ALP activity assay and Alizarin red quantitative detection. The effect of 5 J/cm2 red LEDs on the expression levels of the ALP, Runx2, OCN, OPN and BSP genes and proteins was detected by RT-PCR and western blot, respectively.

Results

Red LEDs at 1, 3, 5, and 7 J/cm2 promoted the proliferation of hSCAPs (P < 0.05). The effects of red LEDs with different light energies on the proliferation of hSCAPs were different at different time points (P < 0.05). On the 7th and 14th days after irradiation, red LEDs promoted the osteogenic differentiation of hSCAPs, and the effect of 5 J/cm2 red LEDs was the most obvious under osteogenic induction culture conditions (P < 0.05). Red LEDs (5 J/cm2) promoted the expression of the ALP, Runx2, OCN, OPN and BSP genes and proteins (P < 0.05).

Conclusion

Red LEDs promoted the proliferation and osteogenic differentiation of hSCAPs.

Keywords

mesenchymal stem cellshuman stem cells from apical papillaphotobiomodulationred LEDcell proliferationalkaline phosphataseosteocalcinosteopontinosteogenic differentiationtissue engineeringstem cell-based therapy
CLC number: R78 Document code: A Article ID: 2096-1456(2022)05-0321-09

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Journal of Prevention and Treatment for Stomatological Diseases
Volume 30 Issue 5,
May 2022
Pages 321-329
DOI: 10.12016/j.issn.2096-1456.2022.05.003

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Cite this article:
SU Y, HOU L, JIANG B, et al. Effects of a red light-emitting diode on the proliferation and osteogenic differentiation of stem cells from the apical papilla. Journal of Prevention and Treatment for Stomatological Diseases, 2022, 30(5): 321-329. https://doi.org/10.12016/j.issn.2096-1456.2022.05.003

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Received: 08 September 2021
Revised: 10 December 2021
Published: 20 May 2022
© 2022 by Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases

This article is licensed under a Creative Commons Attribution 4.0 International License.