AI Chat Paper
Note: Please note that the following content is generated by AMiner AI. SciOpen does not take any responsibility related to this content.
{{lang === 'zh_CN' ? '文章概述' : 'Summary'}}
{{lang === 'en_US' ? '中' : 'Eng'}}
Chat more with AI
Powered by AMiner. Clicking this button will take you away from SciOpen.
Home Journal of Prevention and Treatment for Stomatological Diseases Article
PDF (2.4 MB)
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript AI Chat Paper
Show Outline
Outline
Show full outline
Hide outline
Outline
Show full outline
Hide outline
Basic Study | Publishing Language: Chinese | Open Access

The effect of silencing the endoplasmic reticulum stress-related protein calnexin on the proliferation, invasion, and migration of tongue squamous cell carcinoma cells

qijian ZHONG1, Tingting JIN2, Yu PENG3, Weixiong CHEN2( ), Jinsong LI3( )
Department of Stomatology, University of Chinese Academy Sciences-Shenzhen Hospital, Shenzhen 518106, China
Department of Stomatology, Longgang District Central Hospital, Affliated to Guangzhou University of Traditional Chinese Medicine, Shenzhen 518116, China
Department of Oral and Maxillofacial Surgery, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou 510120, China
Show Author Information

Abstract

Objective

To investigate the effect of silencing the endoplasmic reticulum stress-related protein calnexin on the proliferation, invasion, and migration of tongue squamous cell carcinoma cells.

 Methods

Calnexin siRNA was transfected into SCC-9 and SCC-25 tongue squamous cell carcinoma cells, and the expression of calnexin was detected by qRT-PCR. The silencing effect of calnexin siRNA was further verified by Western blotting. CCK-8 assay was applied to detect the effect of silencing calnexin on the proliferation of tongue squamous cell carcinoma cells; Transwell assay was used to detect the effect of silencing calnexin on the invasion and migration of tongue squamous cell carcinoma cells.

 Results

qRT-PCR showed that calnexin siRNA could effectively downregulate the expression of calnexin. Western blot analysis further confirmed the silencing effect of calnexin siRNA on calnexin. The CCK-8 assay showed that silencing calnexin expression on the 4th and 5th days could inhibit the proliferation of tongue squamous cell carcinoma cells, and the difference was statistically significant (P < 0.01). The Transwell assay showed that knockdown of calnexin could inhibit the invasion and migration of tongue squamous cell carcinoma cells (P < 0.001).

 Conclusion

Knockdown of calnexin can inhibit the proliferation, invasion, and migration of tongue squamous cell carcinoma cells.

Keywords

endoplasmic reticulum stresscalnexintongue squamous cell carcinomaSCC-9 cellsSCC-25 cellsproliferationinvasionmigrationsiRNAtherapeutic target
CLC number: R78 Document code: A Article ID: 2096-1456(2021)08-0535-06

References

【1】
【1】
 
 
Crossref Google Scholar
Journal of Prevention and Treatment for Stomatological Diseases
Volume 29 Issue 8,
August 2021
Pages 535-540
DOI: 10.12016/j.issn.2096-1456.2021.08.005

{{item.num}}

Comments on this article

Go to comment

< Back to all reports

Review Status: {{reviewData.commendedNum}} Commended , {{reviewData.revisionRequiredNum}} Revision Required , {{reviewData.notCommendedNum}} Not Commended Under Peer Review

Review Comment

Cite this Report
Close
Close
Cite this article:
ZHONG q, JIN T, PENG Y, et al. The effect of silencing the endoplasmic reticulum stress-related protein calnexin on the proliferation, invasion, and migration of tongue squamous cell carcinoma cells. Journal of Prevention and Treatment for Stomatological Diseases, 2021, 29(8): 535-540. https://doi.org/10.12016/j.issn.2096-1456.2021.08.005

5

Views

0

Downloads

0

Crossref

0

Scopus
Received: 25 January 2021
Revised: 01 March 2021
Published: 20 August 2021
© 2021 by Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases

This article is licensed under a Creative Commons Attribution 4.0 International License.