Regulation mechanism of rnc gene on Streptococcus mutans environmental tolerance and its mechanism

To study the changes of acid resistance, oxidation resistance and high osmotic pressure resistance of Streptococcus mutans after knockout of rnc gene and its possible regulatory mechanism.

Through PCR ligation mutagenesis, an rnc knockout mutant (Smurnc) was constructed. Acid tolerance, oxidation tolerance and high osmotic pressure tolerance were compared between Smurnc and the wild strain respectively. Real-time RT-PCR was used to verify the changes in expression of stress tolerance related genes at the transcriptional level.

When rnc gene was knocked out, the acid tolerance (χ²=13.464, P=0.001) and oxidation tolerance (χ²=4.505, P=0.048) of Streptococcus mutans was significantly decreased, but the high osmotic pressure tolerance was significantly increased (χ²=11.971, P=0.001). Expression of stress tolerance related genes luxS and ropA (0.64 and 0.51 times expression of the wild strain) had been significantly downregulated (P<0.001). Expression of htrA and brpA (1.56 and 1.80 times expression of the wild strain) had been significantly upregulated (P<0.001).

The deletion of rnc gene affects the expression of the environmental tolerance related genes of Streptococcus mutans, which reduces its acid resistance and oxidation resistance, and enhances its tolerance to hypertonic pressure.