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Basic Study | Publishing Language: Chinese | Open Access

Expression of miR-21, miR-221, and miR-222 in exosomes of CAL27 tongue squamous cell carcinoma cells

Wenxi HUANG1, Ying OUYANG2, Changbo WEI2, Dongsheng YU2( )
Department of Stomatology, Foshan Chancheng District Centre Hospital, Foshan 528000, China
Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China
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Abstract

Objective

To assess the expression of miR-21, miR-221, and miR-222 in exosomes of CAL27 tongue squamous cell carcinoma cells.

Methods

CAL27 tongue squamous cell carcinoma cells and normal human oral keratinocytes (HOKs) were cultured, and then, the cultured supernatant was collected to separate the exosomes. Exosomes were detected by electron microscopy, and the expression levels of miR-21, miR-221, and miR-222 in the exosomes of tongue cancer cells were measured by qRT-PCR.

Results

Exosomes existed in the cultured supernatants of CAL27 cells and HOKs. Additionally, the expression levels of miR-21, miR-221, and miR-222 in the exosomes of CAL27 cells were significantly enhanced compared with those in the HOK exosomes (P < 0.05).

Conclusion

The expression levels of miR-21, miR-221, and miR-222 were markedly enhanced in the exosomes of CAL27 tongue squamous cell carcinoma cells.

Keywords

CarcinomaTongueSquamous cellExosomemiR-21miR-221miR-222qRT-PCR
CLC number: R739.8 Document code: A Article ID: 2096-1456(2018)04-0222-05

References

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Journal of Prevention and Treatment for Stomatological Diseases
Volume 26 Issue 4,
April 2018
Pages 222-226
DOI: 10.12016/j.issn.2096-1456.2018.04.004

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HUANG W, OUYANG Y, WEI C, et al. Expression of miR-21, miR-221, and miR-222 in exosomes of CAL27 tongue squamous cell carcinoma cells. Journal of Prevention and Treatment for Stomatological Diseases, 2018, 26(4): 222-226. https://doi.org/10.12016/j.issn.2096-1456.2018.04.004

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Received: 10 October 2017
Revised: 07 November 2017
Published: 20 April 2018
© 2018 by Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases

This article is licensed under a Creative Commons Attribution 4.0 International License.