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Special Article | Publishing Language: Chinese | Open Access

Effect of Porphyromonas gingivalis lipopolysaccharide on gene expression of inflammatory cytokines in peritoneal macrophages of hyperlipidemic rabbits

Xiaoqing YOU1, Kai LUO1, Yanfen LI1, Chunfang WU1, Fuhua YAN2( )
The affiliated Stomatological Hospital, Fujian Medical University, Key laboratory of Stomatology, Fujian Province University, Fujian Biological Materials Engineering and Technology Center of Stomatology, Fuzhou 350002, China
Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing 210008, China
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Abstract

Objective

To compare the inflammatory reaction of peritoneal macrophage after Pg-LPS stimulated in healthy rabbit and hyperlipidemia rabbit.

Methods

12 New Zealand rabbits were randomly divided into 2 groups with 6 rabbits in each group, and normal diet and high-fat diet were fed to them respectively. The hyperlipidemia model was set up after 6 weeks. The peritoneal macrophage in normal and hyperlipidemia group were isolated and cultured, and then the cells in both groups were respectively divided into 3 groups: control, 1 μg/mL Pg-LPS, and 1 μg/mL E.coli-LPS. After 24 h treatment, the expressions of C-reaction protein (CRP), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α) were detected by realtime PCR.

Results

The levels of CRP, IL-1β, IL-6, IL-8, and TNF-α were higher in hyperlipidemia control group than normal control group. The expressions of inflammatory substances were increased after stimulated by Pg-LPS, and statistically higher in hyperlipidemia rabbit group than normal group (P < 0.05).

Conclusion

Pg-LPS can enhance the mRNA expressions of inflammation related factors in peritoneal macrophage in hyperlipidemia rabbit.

Keywords

Porphyromonas gingivalisLipopolysaccharideHyperlipidemiaPeritoneal macrophageInflammation factorPeriodontitis
CLC number: R781.4 Document code: A Article ID: 2096-1456(2017)11-0687-06

References

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Journal of Prevention and Treatment for Stomatological Diseases
Volume 25 Issue 11,
November 2017
Pages 687-692
DOI: 10.12016/j.issn.2096-1456.2017.11.002

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Cite this article:
YOU X, LUO K, LI Y, et al. Effect of Porphyromonas gingivalis lipopolysaccharide on gene expression of inflammatory cytokines in peritoneal macrophages of hyperlipidemic rabbits. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(11): 687-692. https://doi.org/10.12016/j.issn.2096-1456.2017.11.002

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Received: 15 February 2017
Revised: 23 April 2017
Published: 20 November 2017
© 2017 by Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases

This article is licensed under a Creative Commons Attribution 4.0 International License.