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Special Article | Publishing Language: Chinese | Open Access

Effect of Notch signaling and autophagy on MTA induced differentiation of human dental pulp cells in vitro

Fei HE1, Yuyan ZHENG1( ), Wei CHOU2, Guoquan ZHANG1, Sui MAI3
Department of Stomatology, the Second Clinical Medical College & Shenzhen People's Hospital, Ji'nan University, Shenzhen 518020, China
Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China
Department of Conservative Dentistry and Endodontics Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou 510055, China
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Abstract

Objective

The aim of this study is to investigate the roles of Notch signaling and autophagy on mineral trioxide aggregate (MTA) induced differentiation of human dental pulp cells (hDPCs).

Methods

Third molars from healthy human were collected and hDPCs were isolated by a combined digestion of collagenase Ⅰ and dispaseⅡ. Real time PCR were used to test the mRNA expression levels of alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2) and dentin sialophoprotein (DSPP) in MTA treated hDPCs in different time (24 h, 3 d and 7 d). The mineralization nodules formed by hDPCs with or without MTA treatment were detected by Von Kossa staining. Expressions of Notch1, Jagged1, Hes1, LC3Ⅱ/LC3 Ⅰand p62 in wild type and MTA treated hDPCs were detected by western blotting.

Results

MTA extracted in a concentration of 0.1 mg/mL could promote the differentiation of hDPCs. Compared with that of wild type hDPCs, the expressions of Notch1, Hes1, or Jagged1 and p62 (P＜0.01) in MTA treated hDPCs were significantly increased. MTA treatment showed inhibition effects on autophagy flux similar to Bafilomycin A1, a specific inhibitor of fusion between autophagosomes and lysosomes.

Conclusion

MTA could promote hDPCs differentiation with highly relevant in stimulating Notch1-Jagged1-Hes1 signaling and inhibition of autophagy flux.

Keywords

Mineral trioxide aggregateDental pulp cells (DPCs)DifferentiationNotchAutophagy
CLC number: R780.2 Document code: A Article ID: 2096-1456(2017)07-0414-06

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Journal of Prevention and Treatment for Stomatological Diseases
Volume 25 Issue 7,
July 2017
Pages 414-419
DOI: 10.12016/j.issn.2096-1456.2017.07.002

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Cite this article:
HE F, ZHENG Y, CHOU W, et al. Effect of Notch signaling and autophagy on MTA induced differentiation of human dental pulp cells in vitro. Journal of Prevention and Treatment for Stomatological Diseases, 2017, 25(7): 414-419. https://doi.org/10.12016/j.issn.2096-1456.2017.07.002

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Received: 11 March 2017
Revised: 23 May 2017
Published: 20 July 2017
© 2017 by Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases

This article is licensed under a Creative Commons Attribution 4.0 International License.