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Special Article | Publishing Language: Chinese | Open Access

Effect of Ezrin on epithelial-to-mesenchymal transition in human tongue squamous cell carcinoma with cisplatin-resistance

Zhi-li ZHANG1, Wen-hui LU1, Wei CENG1, Zhang DENG1, Bin ZHOU2, You-yuan WANG2( )
Department of Stomatology, Meizhou People's Hospital, Meizhou Hospital Affiliated to Sun Yat - sen University, Meizhou 514031, China
Department of Stomatology, Sun Yat - sen Memorial Hospital Affiliated to Sun Yat - sen University, Guangzhou 510120, China
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Abstract

Objective

To investigate the effect of Ezrin on epithelial-to-mesenchymal transition(EMT) in human tongue squamous cell carcinoma (TSCC) with cisplatin-resistance.

Methods

A MTT-based method was used to analyse the half maximal inhibitory concentration (IC50) values of TSCC cell line CAL27 and its cisplatin - resistant cell line CAL27/CDDP. Bright-phase microscopic was used to observe the morphological changes. Western blot was used to detect for Vimentin, E-cadherin and Ezrin expression. Transwell assay was used to detect for invasion. After transfected Ezrin with siRNA, Ezrin, Vimentin and E-cadherin expression, as well as invasion, were furthered detected in CAL27/CDDP.

Results

IC50 of CAL27/CDDP increased by 6.8 fold compared to its parent cell line. CAL27 cells with polygonal morphology stimulated by cisplatin trans-differentiated into epithelioid structure with clustered shape and tight connection between the cells whereas CAL27/CDDP showed a mesenchymal phenotype with an elongated morphology. CAL27/CDDP showed upregulated Vimentin, downregulated E-cadherin and upregulated Ezrin expression, as well as increased invasion. After knocking down Ezrin with siRNA, Ezrin and Vimentin were downregulated but E-cadherin was upregulated in CAL27/CDDP, as well as decreased invasion.

Conclusion

Knocking down of Ezrin inhibits epithelial-to-mesenchymal transition and metastasis in TSCC with cisplatin-resistance.

Keywords

EzrinTongue squamous cell carcinomaEpithelial-to-mesenchymal transitionChemo-resistancesiRNA
CLC number: R739.86 Document code: A Article ID: 2096-1456(2016)12-688-07

References

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Journal of Prevention and Treatment for Stomatological Diseases
Volume 24 Issue 12,
December 2016
Pages 688-694
DOI: 10.12016/j.issn.2096-1456.2016.12.002

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ZHANG Z-l, LU W-h, CENG W, et al. Effect of Ezrin on epithelial-to-mesenchymal transition in human tongue squamous cell carcinoma with cisplatin-resistance. Journal of Prevention and Treatment for Stomatological Diseases, 2016, 24(12): 688-694. https://doi.org/10.12016/j.issn.2096-1456.2016.12.002

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Received: 03 May 2016
Revised: 04 June 2016
Published: 20 December 2016
© 2016 by Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases

This article is licensed under a Creative Commons Attribution 4.0 International License.