AI Chat Paper
Note: Please note that the following content is generated by AMiner AI. SciOpen does not take any responsibility related to this content.
{{lang === 'zh_CN' ? '文章概述' : 'Summary'}}
{{lang === 'en_US' ? '中' : 'Eng'}}
Chat more with AI
Powered by AMiner. Clicking this button will take you away from SciOpen.
Home Journal of Prevention and Treatment for Stomatological Diseases Article
PDF (778.8 KB)
Cite
EndNote(RIS) BibTeX
Collect
Submit Manuscript AI Chat Paper
Show Outline
Outline
Show full outline
Hide outline
Outline
Show full outline
Hide outline
Basic Study | Publishing Language: Chinese | Open Access

Study on the role of long non-coding RNA TUG1 in tongue squamous cell carcinoma

Zhi-qiang LI1, Rui ZOU2, Ke-xiong OUYANG2, Zhi-bao BAI3, Wei-jian AI1( )
Department of Oral and Maxillofacial Surgery, Guangdong Provincial Stomatological Hospital, the Affiliated Stomatological Hospital of Southern Medical University, Guangzhou 510280, China
Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University; Guangzhou 510140, China
Department of Stomatology Guangzhou First Peoples′ Hospital, Guangzhou 510180, China
Show Author Information

Abstract

Objective

To study the expression of LncRNA TUG1 in tongue squamous cell carcinoma (TSCC), and explore its function in the development of TSCC.

Methods

Fresh samples of TSCC tissues and corresponding adjacent normal tongue tissues were obtained from 19 cases, and the expressions of TUG1 were detected by Q-PCR. The expression of TUG1 in CAL27 cell line were down-regulated by siRNA technology, and the cell proliferation ability after transfection was analyzed by MTT assay. The expressions of iNOS gene were rated by Q-PCR.

Results

Result of Q-PCR revealed the expressions of LncRNA TUG1 were up-regulated in TSCC tissues compared with the adjacent normal tongue tissues (P＜0.001). Cell proliferation ability was inhibited after down-regulation of TUG1 by siRNA technology, and the inhibition rates of 24/48/72 hour were 14.16%. 16.96% and 21.40% respectively. The relative expression of iNOS in blank/control/experimental groups were 1.000 ± 0.034, 0.974 ± 0.045 and 0.729 ± 0.039 respectively, and iNOS was down- regulated significantly in the experimental group (P = 0.002).

Conclusion

LncRNA TUG1 may have functions as a cancerogenic substance in TSCC via pormoting the expression of iNOS.

Keywords

TongueSquamous cell carcinomaLong non-coding RNATaurine upregulated gene 1Inducible nitric oxide synthase
CLC number: R739.8 Document code: A Article ID: 2096-1456(2016)09-511-04

References

【1】
【1】
 
 
Crossref Google Scholar
Journal of Prevention and Treatment for Stomatological Diseases
Volume 24 Issue 9,
September 2016
Pages 511-514
DOI: 10.12016/j.issn.2096-1456.2016.09.003

{{item.num}}

Comments on this article

Go to comment

< Back to all reports

Review Status: {{reviewData.commendedNum}} Commended , {{reviewData.revisionRequiredNum}} Revision Required , {{reviewData.notCommendedNum}} Not Commended Under Peer Review

Review Comment

Cite this Report
Close
Close
Cite this article:
LI Z-q, ZOU R, OUYANG K-x, et al. Study on the role of long non-coding RNA TUG1 in tongue squamous cell carcinoma. Journal of Prevention and Treatment for Stomatological Diseases, 2016, 24(9): 511-514. https://doi.org/10.12016/j.issn.2096-1456.2016.09.003

298

Views

0

Downloads

0

Crossref

0

Scopus
Received: 24 January 2016
Revised: 05 February 2016
Published: 20 September 2016
© 2016 by Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases

This article is licensed under a Creative Commons Attribution 4.0 International License.