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Basic Study | Publishing Language: Chinese | Open Access

Study of the cellular growth inhibited by tetrandrine with the expression of β-catenin in oral squamous cell carcinoma cell line

Yan-fei CHENG1( ), Yong-rong ZHONG2
Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510110, China
Guangdong Provincial Stomatological & the Affiliated Stomatological Hospital of Southern Medical Universtity, Guangzhou 510280, China
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Abstract

Objective

To study the effect of tetrandrine (TET) on oral squamous cell carcinoma cell line Tca8113 and its influence against the expression of β-catenin.

Methods

CCK-8 method was used to detect the effect of TET on Tca8113 by different dose and time. Western blot assay was used to detect the expression of β-catenin after treatment by TET meanwhile the distribution of the β-catenin treated by TET was detected by the immunofluorescence method.

Results

With the increasing dose of TET, the inhibition rate against Tca8113 was raising (P＜0.05). The half maximal inhibitory concentration (IC50) value of TET against Tca8113 was 64 μmol/L. With this concentration, the inhibition rate against Tca8113 exhibited a time-dependent manner. The results of western blot revealed that the expression of β-catenin was significantly inhibited after treatment by TET (P＜0.05). The results of immunofluorescence method showed that the β-catenin protein mainly located at nucleus in Tca8113, while after treatment by TET, the β-catenin protein mainly located at cellular membrane and cytoplasm in a diffused distribution manner.

Conclusion

Our data suggest that TET can inhibit the cell growth of oral squamous cell carcinoma, and influence the cellular distribution of β-catenin protein in tumor cells.

Keywords

TetrandrineOral squamous cell carcinomaβ-cateninCell growthCell culture
CLC number: R739.8 Document code: A Article ID: 2096-1456(2016)08-464-05

References

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Journal of Prevention and Treatment for Stomatological Diseases
Volume 24 Issue 8,
August 2016
Pages 464-468
DOI: 10.12016/j.issn.2096-1456.2016.08.006

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CHENG Y-f, ZHONG Y-r. Study of the cellular growth inhibited by tetrandrine with the expression of β-catenin in oral squamous cell carcinoma cell line. Journal of Prevention and Treatment for Stomatological Diseases, 2016, 24(8): 464-468. https://doi.org/10.12016/j.issn.2096-1456.2016.08.006

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Received: 15 March 2016
Revised: 28 April 2016
Published: 20 August 2016
© 2016 by Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases

This article is licensed under a Creative Commons Attribution 4.0 International License.