Adsorption mechanism of Pichia pastoris hydrolysate on zearalenone and deoxynivalenol

In order to effectively prevent and remove toxins, using Pichia pastoris as research object. Yeast hydrolysates with different hydrolysis degrees (YH1-YH5) were prepared by controlling the autolysis time and enzymatic hydrolysis time of P. pastoris hydrolysates (YHs) and supplemented with mechanical wall breaking treatment. Using unhydrolyzed dry yeast powder (YH0) as control, the effects of YHs on the adsorption of zearalenone (ZEN) and vomitoxin (DON) under different hydrolysis degrees, addition, and pH conditions were investigated, and the changes in infrared spectra before and after toxin adsorption were analyzed. In addition, the adsorption of ZEN and DON by YHs was studied through in vitro static simulation digestion experiments. The results showed that YH1-YH5 had significantly better adsorption and removal effects on ZEN and DON than YH0 (P<0.05). When YH5 addition was 20 mg/ml and pH was 4.5 and 9.5, respectively, the maximum removal rates of ZEN and DON were 85.48% and 92.95%, respectively. According to infrared spectroscopy analysis, ZEN mainly binds to the outer side of YHs cell wall through hydrophobic interactions with mannans, while DON mainly binds to YHs through hydrogen bonds. In vitro static simulation digestion experiments results showed that YHs can effectively adsorb DON in vivo, but their adsorption effect on ZEN was limited and cannot achieve effective removal.