Microbial diversity in the pit mud of based on MiSeq high-throughput sequencing and pure culture technology

In this study, the microbial diversity of the pit mud of Laohekou Guanghuate Liquor Industry Co., Ltd. was analyzed using MiSeq high-throughput sequencing technology, and the Bacillus contained in it was isolated and identified through pure culture technology. The results showed that the species richness and diversity of bacterial groups in pit mud were significantly higher than those of fungus (P<0.01). A total of 15 dominant bacterial genera were identified, including Sedentibacter (15.62%), Petrimonas (14.58%), and Caloribacterium (10.28%). A total of 12 dominant fungal genera were identified, including Saccharomycopsis (35.40%), Thermoascus (11.86%), Rhizopus (9.81%), and Aspergillus (7.45%). A total of 7 strains of Bacillus were isolated and identified from pit mud using pure cultivation techniques, including 3 strains of Bacillus amyloliquefaciens, 2 strains of Bacillus australimaris, and 2 strains of Bacillus subtilis. The research results provided certain data support for the diversity analysis of pit mud microorganisms and the screening of functional microorganisms.