Advances in modification of Saccharomyces cerevisiae by synthetic biology and genetic manipulation technology

As a classic model eukaryote, Saccharomyces cerevisiae has been continuously playing a core role in industrial biotechnology, leveraged by its convenient genetic manipulation, efficient glycolytic capacity, and complete molecular toolbox. With the rapid development of modern life sciences, the application scenarios of S. cerevisiae have been further expanded in both breadth and depth. The latest research achievements in modifying S. cerevisiae based on genetic engineering, gene editing, metabolic engineering, and synthetic biotechnology over the past five years were systematically reviewed in this paper. The development trends were analyzed from the perspectives of technological innovation and application breakthroughs, and the future development directions of modified S. cerevisiae were prospected, providing a systematic technical route reference for the in-depth development of the biomanufacturing potential of S. cerevisiae.