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Research Article | Open Access

A high-quality haplotype genome of Michelia alba DC reveals differences in methylation patterns and flower characteristics

Sirong Jiang1,2,†, Meiling Zou1,2,†, Chenji Zhang3,†, wanfeng Ma1,2, Chengcai Xia1,2, Zixuan Li1,2, Long Zhao4, Qi Liu1,2, Fen Yu1,2, Dongyi Huang2( ), Zhiqiang Xia1,2( )
Sanya Nanfan Research Institute of Hainan University, Hainan Yazhou Bay Seed Laboratory, Sanya, China
College of Tropical Crops, Hainan University, Haikou, China
China Agricultural University, Beijing, China
Qinghai University, Xining, China

†Sirong Jiang, Meiling Zou and Chenji Zhang contributed equally to this work.

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Abstract

Michelia alba DC is a highly valuable ornamental plant of the Magnoliaceae family. This evergreen tropical tree commonly grows in Southeast Asia and is adored for its delightful fragrance. Our study assembled the M. alba haplotype genome MC and MM by utilizing Nanopore ultralong reads, Pacbio Hifi long reads and parental second-generation data. Moreover, the first methylation map of Magnoliaceae was constructed based on the methylation site data obtained using Nanopore data. Metabolomic datasets were generated from the flowers of three different species to assess variations in pigment and volatile compound accumulation. Finally, transcriptome data were generated to link genomic, methylation, and morphological patterns to reveal the reasons underlying the differences between M. alba and its parental lines in petal color, flower shape, and fragrance. We found that the AP1 and AP2 genes are crucial in M. alba petal formation, while the 4CL, PAL, and C4H genes control petal color. The data generated in this study serve as a foundation for future physiological and biochemical research on M. alba, facilitate the targeted improvement of M. alba varieties, and offer a theoretical basis for molecular research on Michelia L.

Keywords

Michelia LHaplotype genome assemblyDNA methylationAllele genesFlower

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Molecular Horticulture
Volume 4 Issue 2,
June 2024
Article number: 23
DOI: 10.1186/s43897-024-00098-z

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Cite this article:
Jiang S, Zou M, Zhang C, et al. A high-quality haplotype genome of Michelia alba DC reveals differences in methylation patterns and flower characteristics. Molecular Horticulture, 2024, 4(2): 23. https://doi.org/10.1186/s43897-024-00098-z

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Received: 28 September 2023
Accepted: 19 April 2024
Published: 29 May 2024
© The Author(s) 2024.

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