Eleven new species of Trichoderma (Hypocreaceae, Hypocreales) from China

Trichoderma spp. are globally distributed and are considered significant fungal resources. This study presents the discovery of 11 new species of Trichoderma: T. caeruleum, T. gongcheniae, T. graminicola, T. graminis, T. hongkuii, T. parapeberdyi, T. neoguizhouense, T. neohongkuii, T. parahamatum, T. parahongkuii, and T. shaanxiensis. All of these new species were isolated from soils, except for T. caeruleum, T. graminicola, T. graminis, and T. neohongkuii, which were found as endophytes in Poaceae plants. The phylogenetic position of these novel species was determined by analysing the concatenated sequences of the second largest nuclear RNA polymerase subunit encoding gene (rpb2) and the translation elongation factor 1-alpha encoding gene (tef1). The results of the phylogenetic analysis revealed that each new species formed a distinct lineage: T. gongcheniae, T. graminicola, T. graminis, T. neoguizhouense, T. parapeberdyi, and T. shaanxiensis belong to the Harzianum Clade, T. hongkuii, T. parahongkuii, and T. neohongkuii are new members of the Koningii Clade, T. parahamatum belongs to the Hamatum Clade, and T. caeruleum does not fall within any of the named clades. The study also provided a detailed description of the morphology and cultural characteristics of the newly discovered Trichoderma species. The discovery contributes to the advancement of knowledge about Trichoderma species resources in China.