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Original Article | Open Access

Adult dental epithelial stem cell-derived organoids deposit hydroxylapatite biomineral

Hyun-Yi Kim1, Victoria Cooley2,3, Eun-Jung Kim4, Shujin Li4, Jong-Min Lee4, Dina Sheyfer3, Wenjun Liu3, Ophir D. Klein5,6, Derk Joester2 , Han-Sung Jung4 ( )
NGeneS Inc., Ansan-si, Gyeonggi-do, Korea
Department of Materials Science and Engineering, Northwestern University, Evanston, IL, USA
X-ray Science Division, Advanced Photon Source, Argonne National Laboratory, Lemont, IL, USA
Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 FOUR Project, Yonsei University College of Dentistry, Seoul, Korea
Department of Orofacial Sciences and Program in Craniofacial Biology, University of California, San Francisco, CA, USA
Department of Pediatrics, Cedars-Sinai Guerin Children’s, Los Angeles, CA, USA
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Abstract

Ameloblasts are specialized cells derived from the dental epithelium that produce enamel, a hierarchically structured tissue comprised of highly elongated hydroxylapatite (OHAp) crystallites. The unique function of the epithelial cells synthesizing crystallites and assembling them in a mechanically robust structure is not fully elucidated yet, partly due to limitations with in vitro experimental models. Herein, we demonstrate the ability to generate mineralizing dental epithelial organoids (DEOs) from adult dental epithelial stem cells (aDESCs) isolated from mouse incisor tissues. DEOs expressed ameloblast markers, could be maintained for more than five months (11 passages) in vitro in media containing modulators of Wnt, Egf, Bmp, Fgf and Notch signaling pathways, and were amenable to cryostorage. When transplanted underneath murine kidney capsules, organoids produced OHAp crystallites similar in composition, size, and shape to mineralized dental tissues, including some enamel-like elongated crystals. DEOs are thus a powerful in vitro model to study mineralization process by dental epithelium, which can pave the way to understanding amelogenesis and developing regenerative therapy of enamel.

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International Journal of Oral Science
Volume 15,
2023
Article number: 55
DOI: 10.1038/s41368-023-00257-w

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Cite this article:
Kim H-Y, Cooley V, Kim E-J, et al. Adult dental epithelial stem cell-derived organoids deposit hydroxylapatite biomineral. International Journal of Oral Science, 2023, 15: 55. https://doi.org/10.1038/s41368-023-00257-w

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Received: 27 June 2023
Revised: 02 November 2023
Accepted: 02 November 2023
Published: 07 December 2023
© The Author(s) 2023

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