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Research Article | Open Access

Thermal image segmentation in weedy fields via synthetic RGB-trained models and GAN-based cross-modality alignment

Earl Ranarioa, Ismael Mayanjaa, Heesup Yuna, Brian N. Baileyb, J. Mason Earlesa( )
Biological Systems Engineering, UC Davis, Davis, USA
Plant Sciences, UC Davis, Davis, USA
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Abstract

Accurate plant segmentation in thermal imagery remains a significant challenge for high throughput field phenotyping, particularly in outdoor environments where low contrast between plants and weeds and frequent occlusions hinder performance. To address this, we present a framework that leverages synthetic RGB imagery, a limited set of real annotations, and GAN-based cross-modality alignment to enhance semantic segmentation in thermal images. We trained models on 1128 synthetic images containing complex mixtures of crop and weed plants in order to generate image segmentation masks for crop and weed plants. We additionally evaluated the benefit of integrating as few as 20 real, manually segmented field images within the training process using various sampling strategies. When combining all the synthetic images with a few labeled real images, we observed a maximum relative improvement of the mean IoU score of 25% compared to the synthetic-only baseline. Cross-modal alignment was enabled by translating RGB to thermal using CycleGAN-Turbo, allowing robust template matching without calibration. Results demonstrated that combining synthetic data with limited manual annotations and cross-domain translation via generative models can significantly boost segmentation performance in complex field environments for multi-model imagery.

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Plant Phenomics
Article number: 100214

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Cite this article:
Ranario E, Mayanja I, Yun H, et al. Thermal image segmentation in weedy fields via synthetic RGB-trained models and GAN-based cross-modality alignment. Plant Phenomics, 2026, 8(3): 100214. https://doi.org/10.1016/j.plaphe.2026.100214

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Received: 21 July 2025
Revised: 08 April 2026
Accepted: 11 April 2026
Published: 30 April 2026
© 2026 The Authors. Nanjing Agricultural University.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).