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Original Article | Open Access

Modified Liangfu granule exhibits anti-cancer effects in gastric cancer by regulating apoptosis-related proteins and genes

Ya Lia,b,c,1, Lingqun Zhub,1, Fengqin Shia, Ruibai Lia, Xinyi Chena, Zhi Zhengd, Bin Qiue, Li Houa,c( )
Department of Oncology and Hematology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
Key Laboratory of Chinese Internal Medicine of Educational Ministry and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
Department of Environmental Health Sciences, Yale School of Public Health, Yale University, New Haven, CT 06520, USA
Department of Internal Medicine 5th Division, Jiangxi Cancer Hospital, Jiangxi Cancer Center, Nanchang 330029, China
Department of Pharmacology, Yale School of Medicine, Yale University, New Haven, CT 06520, USA

Peer review under responsibility of Beijing University of Chinese Medicine.

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Abstract

Objective

To investigate the anti-cancer effects and mechanism of modified Liangfu granule (MLFG) in BGC-823 gastric cancer cells.

Methods

The drug serum was extracted from abdominal aorta of Wistar rats treated by cyclophosphamide, dioscin, MLFG (high-, medium-, and low-dose), respectively. MTS assay was performed to detect the effect of different concentrations of MLFG and dioscin on cell growth. The effect of MLFG on cellular apoptosis was detected by flow cytometry. Western blot was used to examine Bcl-2 and Akt in BGC-823 cells treated with MLFG. Quantitative real-time polymerase chain reaction assay was performed to determine the expression level of caspase-3, E2F1 and E2F3 genes in cells treated by MLFG- and dioscin-containing serum.

Results

MLFG- and dioscin-containing serum inhibited the cell proliferation of BGC-823 cells. MLFG induced gastric carcinoma cell apoptosis and inhibited the expression of Bcl-2 and Akt in a dose-dependent manner. MLFG also significantly induced the gene expression of caspase-3 and downregulated E2F1 and E2F3 gene expression.

Conclusion

The effect of MLFG and dioscin on inhibiting cell proliferation and induction of apoptosis of gastric cancer cells might be related to the regulation of Bcl-2 and Akt proteins and the expression of caspase-3, E2F1 and E2F3 genes.

Keywords

Modified Liangfu granuleGastric cancerCaspase-3E2F1E2F3

References

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Journal of Traditional Chinese Medical Sciences
Volume 6 Issue 4,
October 2019
Pages 325-330
DOI: 10.1016/j.jtcms.2019.11.002

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Cite this article:
Li Y, Zhu L, Shi F, et al. Modified Liangfu granule exhibits anti-cancer effects in gastric cancer by regulating apoptosis-related proteins and genes. Journal of Traditional Chinese Medical Sciences, 2019, 6(4): 325-330. https://doi.org/10.1016/j.jtcms.2019.11.002

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Received: 14 January 2019
Revised: 13 November 2019
Accepted: 13 November 2019
Published: 27 November 2019
© 2019 Beijing University of Chinese Medicine.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).