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Original Article | Open Access

Effects of an immunosuppressive active component of Periplocae Cortex (Periploca sepium Bge.) on positive selection of thymocytes in vitro

Huifang Denga, Hong Yangb, Dingnan Wanga, Baihui Zenga, Shaohua Shia, Han Yua, Yunzhou Lia, Zongying Xua, Xianggen Zhonga( )
School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
School of Continuing Education, Beijing University of Chinese Medicine, Beijing 100029, China

Peer review under responsibility of Beijing University of Chinese Medicine.

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Abstract

Objective

To determine the effect of an immunosuppressive active component (periploside A) isolated from the stem bark of Periplocae Cortex (Periploca sepium Bge.), a Chinese medicinal herb used in the treatment of rheumatoid arthritis for centuries in China, on positive selection of thymocytes in vitro.

Methods

Female C57BL/6 mice at 6 weeks of age were housed in specific pathogen-free conditions. Double-positive thymocytes from C57BL/6 mice were induced into positive selection in vitro with or without periploside A treatment. Cell viability and expression of CD69, CD4, and CD8 were analyzed by flow cytometry.

Results

Flow cytometric examination of thymocyte populations revealed that the percentage of CD8+ single-positive thymocytes was decreased by periploside A upon differentiation induced by an anti-CD3 antibody. However, the percentage of CD4+ single-positive thymocytes was decreased by periploside A upon differentiation induced by phorbol 12-myristate 13-acetate/ionomycin. Expression of CD69 plays a major role in prohibiting differentiation of thymocytes. Treatment with periploside A decreased CD69 expression in thymocytes.

Conclusion

These results demonstrate that periploside A influences positive selection of thymocytes in vitro.

Keywords

Immunosuppressive active componentPositive selectionThymocytes

References

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Journal of Traditional Chinese Medical Sciences
Volume 5 Issue 2,
April 2018
Pages 161-167
DOI: 10.1016/j.jtcms.2018.03.002

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Cite this article:
Deng H, Yang H, Wang D, et al. Effects of an immunosuppressive active component of Periplocae Cortex (Periploca sepium Bge.) on positive selection of thymocytes in vitro. Journal of Traditional Chinese Medical Sciences, 2018, 5(2): 161-167. https://doi.org/10.1016/j.jtcms.2018.03.002

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Received: 10 January 2018
Revised: 10 March 2018
Accepted: 20 March 2018
Published: 28 March 2018
© 2018 Beijing University of Chinese Medicine.

This is an open access article under the CCBY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).