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Journal of Neurorestoratology 2023, 11(3): 100067 https://doi.org/10.1016/j.jnrt.2023.100067
Original Research | Open Access | Issue | Published: 05 July 2023

A novel therapeutic approach of ultrasound stimulation to restore forelimb functions following cervical cord injury in rats

Show Author's Information Rakib Uddin Ahmeda,  Monzurul Alama,†( ),  Shuai Lia,  Poornima Palanisamya,  Hui Zhongb,  Yong-Ping Zhenga,†( )
Department of Biomedical Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China
Department of Neurobiology, University of California, Los Angeles, CA 90095, United States

† These authors contributed equally to this work.

Keywords:
Neuromodulation, Non-invasive, LIPUS, Cervical cord injury, Buspirone
Cite this article:
Ahmed RU, Alam M, Li S, et al. A novel therapeutic approach of ultrasound stimulation to restore forelimb functions following cervical cord injury in rats. Journal of Neurorestoratology, 2023, 11(3): 100067. https://doi.org/10.1016/j.jnrt.2023.100067
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Abstract Full text About this article

Low-intensity pulsed ultrasound (LIPUS) stimulation has shown promising results in neurorehabilitation following a traumatic injury in brain and peripheral nerves. However, the effects of LIPUS stimulation in the injured neural circuit after spinal cord injury (SCI) are still unknown. We investigated the effects of LIPUS on forelimb functions in chronic cervical cord injured rats with and without a serotonergic agonist drug, Buspirone treatment. Twenty-six rats were trained for forelimb reaching and grasping followed by C4 dorsal funiculi crush injury. To deliver LIPUS, a silicon-coated ultrasound disc was implanted above the cervical cord and electromyography electrodes were implanted into forelimb muscles. In two cohorts (LIPUS and LIPUS + Buspirone combined) rats were tested pre-, with- and post-ultrasound stimulation. In LIPUS group rats, fore-limb reaching and grasping success rates first increased and then dropped after 3 weeks while for combination of drug and LIPUS stimulation the score continued to increase. Furthermore, LIPUS stimulation alone did not result in any significant improvement of grip strength compared to the control and combined groups. The findings of this study indicated the potential of LIPUS in SCI recovery and offer a future research direction of a new neuromodulation method.


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About this article

A novel therapeutic approach of ultrasound stimulation to restore forelimb functions following cervical cord injury in rats

Show Author's information Rakib Uddin Ahmeda, Monzurul Alama,†( ), Shuai Lia, Poornima Palanisamya, Hui Zhongb, Yong-Ping Zhenga,†( )
Department of Biomedical Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China
Department of Neurobiology, University of California, Los Angeles, CA 90095, United States

† These authors contributed equally to this work.

Abstract

Low-intensity pulsed ultrasound (LIPUS) stimulation has shown promising results in neurorehabilitation following a traumatic injury in brain and peripheral nerves. However, the effects of LIPUS stimulation in the injured neural circuit after spinal cord injury (SCI) are still unknown. We investigated the effects of LIPUS on forelimb functions in chronic cervical cord injured rats with and without a serotonergic agonist drug, Buspirone treatment. Twenty-six rats were trained for forelimb reaching and grasping followed by C4 dorsal funiculi crush injury. To deliver LIPUS, a silicon-coated ultrasound disc was implanted above the cervical cord and electromyography electrodes were implanted into forelimb muscles. In two cohorts (LIPUS and LIPUS + Buspirone combined) rats were tested pre-, with- and post-ultrasound stimulation. In LIPUS group rats, fore-limb reaching and grasping success rates first increased and then dropped after 3 weeks while for combination of drug and LIPUS stimulation the score continued to increase. Furthermore, LIPUS stimulation alone did not result in any significant improvement of grip strength compared to the control and combined groups. The findings of this study indicated the potential of LIPUS in SCI recovery and offer a future research direction of a new neuromodulation method.

Keywords: Neuromodulation, Non-invasive, LIPUS, Cervical cord injury, Buspirone

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Publication history

Received: 18 November 2022
Revised: 08 June 2023
Accepted: 13 June 2023
Published: 05 July 2023
Issue date: September 2023

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© Published by Elsevier Ltd on behalf of Tsinghua University Press.

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This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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