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Original Research | Open Access

Transcriptome alterations and therapeutic drugs in different organs after spinal cord injury based on integrated bioinformatic analysis

Department of Neurosurgery, National Center for Neurological Disorders, Neurosurgical Institute of Fudan University, Shanghai Clinical Medical Center of Neurosurgery, Shanghai Key Laboratory of Brain Function and Restoration and Neural Regeneration, Huashan Hospital, Fudan University, Shanghai 200040, China
Department of Nursing, Huashan Hospital, Fudan University, Shanghai 200032, China
Department of Neurosurgery, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China

1 These authors contributed equally to this work.

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Abstract

Objective

To identify transcriptomic alterations and therapeutic drugs in different organs after spinal cord injury via comprehensive bioinformatics analyses.

Methods

RNA-sequencing data of the soleus muscle, bladder, liver, raphe, and sensorimotor cortex areas in various rat spinal cord injury models were obtained from the Gene Expression Omnibus database for bioinformatics analysis. Then, the common pathways and biological pathway changes in multiple organs were identified.

Results

By comparing the enrichment results of differentially expressed genes, inflammatory response and lipid metabolism were found to be significantly altered in multiple organs. The MAPK signaling pathway was a key pathway in the abovementioned biological processes. In addition, autonomous repair was observed in each organ. Finally, pharmacological analysis showed that coenzyme A might be an effective drug.

Conclusion

Coenzyme A is a candidate treatment drug for spinal cord injury. Hence, in addition to the current mechanisms targeted by anti-inflammatory approaches, lipid metabolism can also be a treatment target for spinal cord injury.

Keywords

InflammationSpinal cord injuryLipid metabolismDifferent organsGene expression profiles

Electronic Supplementary Material

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jnrt-11-2-100056-esm1.docx (5.6 MB)

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Journal of Neurorestoratology
Volume 11 Issue 2,
June 2023
Article number: 100056
DOI: 10.1016/j.jnrt.2023.100056
Cite this article:
Dong H, Shi D, Bao Y, et al. Transcriptome alterations and therapeutic drugs in different organs after spinal cord injury based on integrated bioinformatic analysis. Journal of Neurorestoratology, 2023, 11(2): 100056. https://doi.org/10.1016/j.jnrt.2023.100056

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Received: 11 February 2023
Revised: 27 March 2023
Accepted: 17 April 2023
Published: 09 May 2023
© 2023 The Authors.

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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