Highlights
• Zhonghua 11 and Taichung 65 exhibit distinct physiological and molecular responses to brassinosteroids despite their close japonica relationship.
• A near-isogenic line, d61-zh11, was developed to transfer the classical brassinosteroid (BR)-insensitive mutation into a modern functional genomics background.
• d61-zh11 provides a standardized reference mutant for the reproducible and system-level dissection of BR signaling in rice.
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