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Letter | Open Access

An on-site visual assay detecting Langya henipavirus based on recombinase polymerase amplification technology

Lin Cheng1,2,*, Haili Zhang3,*, Xiaoai Zhang4,*, Pei Huang3, Xinlan Chen1,2, Fangxu Li1, Wujian Li5,6,7, Wei Liu4( ), Hualei Wang3( ), Sandra Chiu5,6,7( ), Zengguo Cao1( )
State Key Laboratory of Virology and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China
University of Chinese Academy of Sciences, Beijing 100190, China
State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases/Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China
Beijing Institute of Microbiology and Epidemiology, Beijing 100101, China
Department of Laboratory Medicine, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230031, China
Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China
Key Laboratory of Anhui Province for Emerging and Reemerging Infectious Diseases, Hefei 230026, China

* These authors contributed equally to this study.

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Highlights

• A visual assay of Langya henipavirus (LayV) nucleic acids was developed based on recombinase polymerase amplification technology (RPA), combined with an immunochromatographic test device.

• The detection limit of our assay reaches 1.22 copies μL-1, with no observed cross-reactivity with other henipaviruses.

• This assay is well-suited for the requirements of point-of-care and field detection and has the potential for broader application.

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Journal of Integrative Agriculture (JIA)
Volume 25 Issue 7,
July 2026
Pages 3099-3101
DOI: 10.1016/j.jia.2025.12.008

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Cite this article:
Cheng L, Zhang H, Zhang X, et al. An on-site visual assay detecting Langya henipavirus based on recombinase polymerase amplification technology. Journal of Integrative Agriculture (JIA), 2026, 25(7): 3099-3101. https://doi.org/10.1016/j.jia.2025.12.008

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Received: 25 May 2025
Revised: 07 October 2025
Accepted: 31 October 2025
Published: 05 December 2025
© 2026 CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer review under responsibility of Editorial Board of Journal of Integrative Agriculture.