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Research Article | Open Access

Optimizing maize yield and kernel quality via leguminous green manure intercropping with deficit irrigation in arid agroecosystem

Diaoliang Zhang1Yunyou Nan2Zhilong Fan1,2( )Qiang Chai1,2( )Gary Y. Gan1,3( )Wen Yin1,2Falong Hu2
State Key Laboratory of Arid Land Crop Science, Gansu Agricultural University, Lanzhou 730070, China
College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China
College of Life and Environmental Sciences, Wenzhou University, Wenzhou 325035, China
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Highlights

• In leguminous green manure intercropping system, 15% deficit irrigation can achieve maize yield equivalent to conventional irrigation.

• It also enhanced kernel quality and sustained high levels of protein and nutrients under deficit irrigation.

• These improvements were attributed to an increased net photosynthetic rate and a greater soil nitrogen supply.

Abstract

Intercropping with leguminous green manure represents a sustainable approach to enhance agroecosystem resilience through improved soil fertility and resource-use efficiency. However, the synergistic mechanisms between leguminous green manure intercropping and regulated deficit irrigation in maintaining maize yield stability and enhancing kernel profiles under arid conditions remain inadequately understood. A three-year (2021–2023) split-plot field experiment incorporated main plots consisting of three green manure incorporation practices: full green manure incorporation (M||V-P), green manure stubble retention (M||V-R), and maize without green manure (maize sole cropping, SM); while split plots comprised three irrigation regimes: conventional (I3; 400 mm), 15% deficit (I2; 340 mm), and 30% deficit (I1; 280 mm). The study examined maize grain yield, kernel quality (protein, fat, starch, and essential amino acid content), net photosynthetic rate (Pn) of maize, and soil nitrate-ammonium nitrogen content. M||V-P and M||V-R increased maize grain yield compared to SM, with M||V-P producing 5.7% higher yields than M||V-R. Notably, M||V-PI2 achieved comparable yield to M||V-PI3 while reducing irrigation by 15%, demonstrating an 18.3% yield increase over SMI3. M||V-P and M||V-R enhanced kernel quality compared to SM, exhibiting higher protein, fat, starch, and essential amino acid content. Decreased irrigation led to increased kernel protein content but reduced fat and starch contents. The kernel protein content under M||V-PI2 showed no significant difference from M||V-PI1, while maintaining fat, starch, and essential amino acid content similar to M||V-PI3. M||V-PI2 improved all kernel quality parameters relative to SMI3. These enhancements primarily resulted from maize intercropped with leguminous green manure in combination with 15% deficit irrigation, which increased maize Pn by 14.3%, and elevated soil nitrate-ammonium nitrogen by 12.5 and 5.2%, respectively. These findings demonstrate a scalable approach for sustainable maize production though the integration of leguminous green manure intercropping in water-limited regions.

References

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Journal of Integrative Agriculture (JIA)
Pages 3017-3030

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Cite this article:
Zhang D, Nan Y, Fan Z, et al. Optimizing maize yield and kernel quality via leguminous green manure intercropping with deficit irrigation in arid agroecosystem. Journal of Integrative Agriculture (JIA), 2026, 25(7): 3017-3030. https://doi.org/10.1016/j.jia.2025.09.011

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Received: 21 April 2025
Revised: 26 June 2025
Accepted: 08 August 2025
Published: 09 September 2025
© 2026 CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer review under responsibility of Editorial Board of Journal of Integrative Agriculture.