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Point-of-care antigen detection for porcine deltacoronavirus: Colloidal gold and fluorescent immunochromatographic test strips

Zezhao Cao1, Junchao Shi1, Ruijie Hu1, Jun Xue1, Gaili Wang2, Zi Li1, Huabo Yu1, Wei Liu1, Wenqi He1, Hualei Wang1, Haili Zhang1( ), Yungang Lan1( )
State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases/Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China
Jilin Academy of Animal Husbandry and Veterinary Medicine, Changchun 130062, China
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Highlights

  • By conjugating the same anti-N monoclonal antibody (mAb4-mAb1) with colloidal gold or fluorescent microspheres, this study developed two rapid point-of-care antigen immunochromatographic strips for the detection of porcine deltacoronavirus.

  • The fluorescent microsphere-based lateral flow test strip demonstrated a sensitivity of 101.7 TCID50/0.1 mL, which is fourfold higher than that of the colloidal gold-based assay.

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Journal of Integrative Agriculture (JIA)
Volume 25 Issue 1,
January 2026
Pages 394-397
DOI: 10.1016/j.jia.2025.08.001

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Cite this article:
Cao Z, Shi J, Hu R, et al. Point-of-care antigen detection for porcine deltacoronavirus: Colloidal gold and fluorescent immunochromatographic test strips. Journal of Integrative Agriculture (JIA), 2026, 25(1): 394-397. https://doi.org/10.1016/j.jia.2025.08.001

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Received: 06 March 2025
Revised: 21 June 2025
Accepted: 12 July 2025
Published: 05 August 2025
© 2026, Chinese Academy of Agricultural Sciences (CAAS). All rights reserved.