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Letter | Open Access

A rapid and visual detection method for porcine deltacoronavirus with single-copy sensitivity based on the CRISPR/Cas12a assay

Zezhao Cao1,*, Zi Li1,*, Kaikai Jin1, Zanheng Huang1, Ruijie Hu1, Junchao Shi1, Huabo Yu1, Gaili Wang2, Wenqi He1, Hualei Wang1, Yungang Lan1( ), Haili Zhang1( )
State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China
Jilin Academy of Animal Husbandry and Veterinary Medicine, Changchun 130062, China

* These authors contributed equally to this study.

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Highlights

• By integrating RT-RAA with CRISPR/Cas12a, a portable, one-pot visual detection system was engineered, significantly minimizing the potential for false positives caused by aerosol contamination.

• RT-RAA-CRISPR/Cas12a assay can complete the detection within 40 min, with a sensitivity as high as 3.9 copies µL–1 for single copy.

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Journal of Integrative Agriculture (JIA)
Volume 25 Issue 4,
April 2026
Pages 1736-1739
DOI: 10.1016/j.jia.2025.07.030

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Cite this article:
Cao Z, Li Z, Jin K, et al. A rapid and visual detection method for porcine deltacoronavirus with single-copy sensitivity based on the CRISPR/Cas12a assay. Journal of Integrative Agriculture (JIA), 2026, 25(4): 1736-1739. https://doi.org/10.1016/j.jia.2025.07.030

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Received: 30 April 2025
Revised: 16 June 2025
Accepted: 26 June 2025
Published: 29 July 2025
© 2026 CAAS.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer review under responsibility of Editorial Board of Journal of Integrative Agriculture.