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Efficient breeding of high oleic rice cultivar by editing OsFAD2-1 via CRISPR/Cas9

Yingying Wu1,2, Zhihui Chen2,3,4, Chan Wang1,2, Yang Xu1,2,3,4, Xia Li2,3,4, Jianping Zhu2,3,4, Xiaoli Tan1, Jie Yang1,2,3,4( )
School of Life Sciences, Jiangsu University, Zhenjiang 212001, China
Key Laboratory of Germplasm Innovation in Downstream of Huaihe River, Ministry of Agriculture and Rural Affairs/Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Zhongshan Biological Breeding Laboratory, Nanjing 210014, China
Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China
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Highlights

  • CRISPR-editing OsFAD2-1 boosts oleic acid and stabilizes rice bran oil.

  • Edited lines maintain key agronomic traits of breeding-ready germplasm.

  • Gene editing accelerates high-oleic breeding in Jiangsu japonica rice.

References

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Journal of Integrative Agriculture (JIA)
Volume 24 Issue 12,
December 2025
Pages 4810-4814
DOI: 10.1016/j.jia.2025.04.037

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Wu Y, Chen Z, Wang C, et al. Efficient breeding of high oleic rice cultivar by editing OsFAD2-1 via CRISPR/Cas9. Journal of Integrative Agriculture (JIA), 2025, 24(12): 4810-4814. https://doi.org/10.1016/j.jia.2025.04.037

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Received: 13 January 2025
Revised: 18 March 2025
Accepted: 09 April 2025
Published: 25 April 2025
© 2025, Chinese Academy of Agricultural Sciences (CAAS). All rights reserved.