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A transient transformation method for pre-screening gRNAs in CRISPR/Cas gene editing

Jinghui Xu1, Xiaocui Yan4, Yan Yu1, Hang Zhao2,3, Menghan Liu2, Ye Wang2, Peng Wang2, Hongying Duan1( ), Xiaoyang Ge2( ), Jingjing Zhan2( )
Henan Normal University Research Base of National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Xinxiang 453000, China
National Key Laboratory of Cotton Bio-breeding and Integrated Utilization/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China
College of Life Sciences, Qufu Normal University, Qufu 273100, China
State Key Laboratory of North China Crop Improvement and Regulation Key Germplasm Resources in North China, Ministry of Education/College of Agronomy, Hebei Agricultural University, Baoding 071001, China
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Highlights

  • This method rapidly evaluates the efficiency of gRNA sequences for genome editing.

  • This method confirms the indicator effect of transient conversion on stable transformations.

  • This approach reduces costs and time.

References

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Journal of Integrative Agriculture (JIA)
Volume 24 Issue 11,
November 2025
Pages 4451-4455
DOI: 10.1016/j.jia.2025.04.007

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Cite this article:
Xu J, Yan X, Yu Y, et al. A transient transformation method for pre-screening gRNAs in CRISPR/Cas gene editing. Journal of Integrative Agriculture (JIA), 2025, 24(11): 4451-4455. https://doi.org/10.1016/j.jia.2025.04.007

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Received: 13 January 2025
Revised: 05 March 2025
Accepted: 13 March 2025
Published: 03 April 2025
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