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A one-pot Cas12i3/Cas13d-based assay for rapid portable identification of genotypes I and II African swine fever viruses

Zhe Wang1,2, Haili Wang1,2, Xinghui Yan3,5, Jin Li3,5, Yu Wang3,5, Guosong Qin3,6,7, Wenbo Sun8, Yanfang Wang4( ), Gaiping Zhang1,2,9 ( ), Jianguo Zhao3,5,6,7( )
Longhu Laboratory of Advanced Immunology, Zhengzhou 450046, China
Henan Key Laboratory of Immunobiology, Zhengzhou 450001, China
Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Savaid Medical School, University of Chinese Academy of Sciences, Beijing 100049, China
Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China
Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China
Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China
School of Advanced Agricultural Sciences, Peking University, Beijing 100871, China
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Highlights

  • A one-pot Cas12i3/Cas13d-based assay is developed to discriminate between ASFV genotype I and II.

  • This method demonstrates complete concordance with the PCR-sequencing method within 45 min.

References

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Journal of Integrative Agriculture (JIA)
Volume 24 Issue 11,
November 2025
Pages 4456-4459
DOI: 10.1016/j.jia.2025.02.013

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Wang Z, Wang H, Yan X, et al. A one-pot Cas12i3/Cas13d-based assay for rapid portable identification of genotypes I and II African swine fever viruses. Journal of Integrative Agriculture (JIA), 2025, 24(11): 4456-4459. https://doi.org/10.1016/j.jia.2025.02.013

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Received: 03 November 2024
Revised: 12 December 2024
Accepted: 04 January 2025
Published: 12 February 2025
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