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On-site visual detection of Nipah virus combining a reverse transcription recombinase-aided amplification with a lateral-flow dipstick assay

Kaikai Jin1, Junjie Zhao1,3, Huanxin Chen1, Zimo Zhang1, Zengguo Cao2, Zanheng Huang1, Hao Li1, Yongsai Liu1, Lisi Ai1, Yufei Liu1, Changqi Fan4, Yuanyuan Li1, Pei Huang1( ), Hualei Wang1( ), Haili Zhang1( )
State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases/Key Laboratory for Zoonosis Research of the Ministry of Education/Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China
Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China
National/WOAH Reference Laboratory for Classical Swine Fever, China Institute of Veterinary Drug Control, Beijing 100081, China
Zhili College, Tsinghua University, Beijing 100084, China
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Highlights

• The RT-RAA-VF assay developed for the NiV P gene can perform rapid detection of NiV within 20 min at 42°C with high specificity.

• This assay is capable of attaining sensitivity to a single copy of NiV RNA transcripts.

• This assay effectively avoids false positives caused by aerosol contamination with a sealed disposable nucleic acid visualization test paper device.

References

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Journal of Integrative Agriculture (JIA)
Volume 24 Issue 2,
February 2025
Pages 790-794
DOI: 10.1016/j.jia.2024.11.018

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Cite this article:
Jin K, Zhao J, Chen H, et al. On-site visual detection of Nipah virus combining a reverse transcription recombinase-aided amplification with a lateral-flow dipstick assay. Journal of Integrative Agriculture (JIA), 2025, 24(2): 790-794. https://doi.org/10.1016/j.jia.2024.11.018

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Received: 19 April 2024
Accepted: 24 September 2024
Published: 20 February 2025
© 2025, Chinese Academy of Agricultural Sciences (CAAS). All rights reserved.